Mouse IL-7 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL7
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	3.20 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 119%
Average Recovery	1.01

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	3.20 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 119%
Average Recovery	1.01
Intra Precision	3.4% – 3.5%
Inter-Precision	3.8% – 4.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-7 monoclonal antibody Mouse IL-7 freeze-dried standard IL-7 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-7 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-7 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-7 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL7

IL7 Target Infomation Overview

Target Symbol: IL7, interleukin 7
Gene Groups: Interleukins
Alias: IL-7

IL7, interleukin 7 Target Infomation by Species

HumanMouseRat

Human IL7 Target Information

Target Symbol: IL7, interleukin 7
Alias:
- IL-7
- IL7 nirs variant 6
NCBI_Gene: 3574
UniProtKB: P13232

Human IL7 Predicted Functions

Enables cytokine activity. Involved in cytokine-mediated signaling pathway; positive regulation of chemokine production; and positive regulation of cytokine-mediated signaling pathway. Located in extracellular region. Part of collagen-containing extracellular matrix. Implicated in epidermodysplasia verruciformis. Biomarker of Alzheimer’s disease and COVID-19.

Mouse Il7 Target Information

Target Symbol: Il7, interleukin 7
Alias:
- A630026I06Rik
- heart, lung and blood 368
- hlb368
- Il-7
- RIKEN cDNA A630026I06 gene
NCBI_Gene: 16196

Mouse Il7 Predicted Functions

Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of lymphocyte activation; and tissue homeostasis. Located in extracellular space. Is expressed in several structures, including brain; branchial arch; hemolymphoid system; intestine; and lymphatic system. Human ortholog(s) of this gene implicated in epidermodysplasia verruciformis. Orthologous to human IL7 (interleukin 7).

Rat Il7 Target Information

Target Symbol: Il7, interleukin 7
Alias:
- IL-7
- interleukin-7
NCBI_Gene: 25647

Rat Il7 Predicted Functions

Predicted to enable cytokine activity and growth factor activity. Involved in cellular response to hypoxia and response to X-ray. Predicted to be located in extracellular space. Used to study high grade glioma. Biomarker of periodontal disease. Human ortholog(s) of this gene implicated in epidermodysplasia verruciformis. Orthologous to human IL7 (interleukin 7).