Mouse IL-7 ELISA Kit

$350.00$450.00

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TARGETSIL7
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity3.20 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 119%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity3.20 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 119%
Average Recovery1.01
Intra Precision3.4% – 3.5%
Inter-Precision3.8% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-7 monoclonal antibody
Mouse IL-7 freeze-dried standard
IL-7 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-7 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-7 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-7 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL7

IL7 Target Infomation Overview

  • Target Symbol: IL7, interleukin 7
  • Gene Groups: Interleukins
  • Alias: IL-7

IL7, interleukin 7 Target Infomation by Species

HumanMouseRat

Human IL7 Target Information

Human IL7 Predicted Functions

Enables cytokine activity. Involved in cytokine-mediated signaling pathway; positive regulation of chemokine production; and positive regulation of cytokine-mediated signaling pathway. Located in extracellular region. Part of collagen-containing extracellular matrix. Implicated in epidermodysplasia verruciformis. Biomarker of Alzheimer’s disease and COVID-19.

Mouse Il7 Target Information

Mouse Il7 Predicted Functions

Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of lymphocyte activation; and tissue homeostasis. Located in extracellular space. Is expressed in several structures, including brain; branchial arch; hemolymphoid system; intestine; and lymphatic system. Human ortholog(s) of this gene implicated in epidermodysplasia verruciformis. Orthologous to human IL7 (interleukin 7).

Rat Il7 Target Information

Rat Il7 Predicted Functions

Predicted to enable cytokine activity and growth factor activity. Involved in cellular response to hypoxia and response to X-ray. Predicted to be located in extracellular space. Used to study high grade glioma. Biomarker of periodontal disease. Human ortholog(s) of this gene implicated in epidermodysplasia verruciformis. Orthologous to human IL7 (interleukin 7).

IL7 Target News

    4.6
    Transient regulatory-T-cell interruption promotes skin-resident memory T cells mediated tumor protection
    Scientific Reports
    7.5
    Angelica sinensis polysaccharides ameliorated 5-Fluorouracil-induced damage of early B cell progenitors by alleviating oxidative stress of IL-7 producing mesenchymal stem and progenitor cells
    BIOMEDICINE & PHARMACOTHERAPY
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Transplantation

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