Mouse PD-1 ELISA Kit

$350.00$450.00

TARGETS PDCD1
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 80 μL 1×Assay Buffer and 20 μL sample
Sensitivity 1.61 pg/mL
Range 78.13 pg/mL – 5000 pg/mL
Assay Time 3.5 h
Recovery 88% – 126%
Average Recovery 1.03
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 80 μL 1×Assay Buffer and 20 μL sample
Sensitivity 1.61 pg/mL
Range 78.13 pg/mL – 5000 pg/mL
Assay Time 3.5 h
Recovery 88% – 126%
Average Recovery 1.03
Intra Precision 1.9% – 2.3%
Inter-Precision 2.6% – 3.0%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PD-1 monoclonal antibody
Mouse PD-1 freeze-dried standard
PD-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse PD-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PD-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PD-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PDCD1

PDCD1 Target Infomation Overview

  • Target Symbol: PDCD1, programmed cell death 1
  • Gene Groups: CD molecules; V-set domain containing
  • Alias: CD279; PD1; hSLE1; PD-1
  • Previous Names: SLEB2
  • Alias Names: systemic lupus erythematosus susceptibility 2

PDCD1, programmed cell death 1 Target Infomation by Species

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Human PDCD1 Target Information

  • Target Symbol: PDCD1, programmed cell death 1
  • Alias:
    • CD279
    • hPD-1
    • hPD-l
    • hSLE1
    • PD-1
    • PD1
    • programmed cell death protein 1
    • SLEB2
    • systemic lupus erythematosus susceptibility 2
  • NCBI_Gene: 5133
  • UniProtKB: Q15116

Human PDCD1 Predicted Functions

Involved in positive regulation of T cell apoptotic process. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Biomarker of several diseases, including Cryptococcal meningitis; anogenital venereal wart; cervix uteri carcinoma in situ; liver disease (multiple); and tuberculosis (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Pdcd1 Target Information

Mouse Pdcd1 Predicted Functions

Involved in negative regulation of immune response. Acts upstream of or within negative regulation of apoptotic process; negative regulation of tolerance induction; and positive regulation of apoptotic process. Located in external side of plasma membrane. Is expressed in retina. Used to study dilated cardiomyopathy and systemic lupus erythematosus. Human ortholog(s) of this gene implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Orthologous to human PDCD1 (programmed cell death 1).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Pdcd1 Target Information

  • Target Symbol: Pdcd1, programmed cell death 1
  • Alias:
    • LOC102550808
    • LOC301626
    • programmed cell death protein 1
    • programmed cell death protein 1-like
  • NCBI_Gene: 301626
  • UniProtKB: D3ZIN8

Rat Pdcd1 Predicted Functions

Predicted to be involved in negative regulation of immune response and positive regulation of T cell apoptotic process. Predicted to act upstream of or within negative regulation of apoptotic process; negative regulation of tolerance induction; and positive regulation of apoptotic process. Located in plasma membrane. Biomarker of anti-basement membrane glomerulonephritis; periodontitis; and pre-eclampsia. Human ortholog(s) of this gene implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Orthologous to human PDCD1 (programmed cell death 1).

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PDCD1 Target News

[catlist tags=”pdcd1″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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