Mouse PDGF-BB ELISA Kit

$299.00 – $419.00

DATASHEET

Related Target	PDGFB
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.05 pg/mL
Array Range	23.44 pg/mL – 1500 pg/mL
Assay Time	3.5 h
Recovery	92% – 122%
Average Recovery	108%

SKU: EK2137 Category: ELISA Kit

ELISA Kit Detail Information

Related Target	PDGFB
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.05 pg/mL
Array Range	23.44 pg/mL – 1500 pg/mL
Assay Time	3.5 h
Recovery	92% – 122%
Average Recovery	108%
Intra Precision	2.5% – 5.0%
Inter Precision	3.3% - 5.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PDGF-BB monoclonal antibody Mouse PDGF-BB freeze-dried standard Mouse PDGF-BB detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse PDGF-BB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PDGF-BB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PDGF-BB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

PDGFB

PDGFB Target Infomation Overview

Target Symbol: PDGFB, platelet derived growth factor subunit B
Alias: SSV
Previous Names: SIS
Alias Names: oncogene SIS; becaplermin; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor beta polypeptide

PDGFB, platelet derived growth factor subunit B Target Infomation by Species

Human
Mouse
Rat

Human PDGFB Target Information

Target Symbol: PDGFB, platelet derived growth factor subunit B
Alias:
- becaplermin
- c-sis
- FLJ12858
- IBGC5
- PDGF subunit B
- PDGF-2
- PDGF, B chain
- PDGF2
- platelet-derived growth factor 2
- platelet-derived growth factor B chain
- platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
- platelet-derived growth factor subunit B
- platelet-derived growth factor, B chain
- Platelet-derived growth factor, beta polypeptide (oncogene SIS)
- proto-oncogene c-Sis
- SIS
- SSV
NCBI_Gene: 5155
UniProtKB: P01127

Human PDGFB Predicted Functions

Enables several functions, including platelet-derived growth factor binding activity; protein dimerization activity; and receptor ligand activity. Involved in several processes, including positive regulation of cell migration; positive regulation of cell population proliferation; and positive regulation of phosphorylation. Located in cell surface. Implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Biomarker of several diseases, including Kuhnt-Junius degeneration; bone disease (multiple); clear cell renal cell carcinoma; diabetic retinopathy (multiple); and reproductive organ cancer (multiple).

Mouse Pdgfb Target Information

Target Symbol: Pdgfb, platelet derived growth factor, B polypeptide
Alias:
- PDGF-B
- simian sarcoma oncogene
- Sis
NCBI_Gene: 18591

Mouse Pdgfb Predicted Functions

Predicted to enable several functions, including platelet-derived growth factor binding activity; protein dimerization activity; and receptor ligand activity. Involved in several processes, including metanephric glomerular mesangium development; paracrine signaling; and positive regulation of glomerular filtration. Acts upstream of or within several processes, including animal organ development; nervous system development; and regulation of cell migration. Predicted to be located in several cellular components, including basolateral plasma membrane; dendrite; and neuronal cell body. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; cardiovascular system; extraembryonic component; female reproductive system; and nervous system. Human ortholog(s) of this gene implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Orthologous to human PDGFB (platelet derived growth factor subunit B).

Rat Pdgfb Target Information

Target Symbol: Pdgfb, platelet derived growth factor subunit B
Alias:
- c-sis
- pdgf protein
- PDGF subunit B
- PDGF-2
- platelet derived growth factor-bb
- platelet derived growth factor, B polypeptide
- Platelet-derived growth factor
- platelet-derived growth factor B chain
- platelet-derived growth factor beta polypeptide
- platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
- Platelet-derived growth factor same as Sis (Simian sarcoma viral oncogene homologue c-sis)
- platelet-derived growth factor subunit B
- Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis)
- SIS
NCBI_Gene: 24628
- UniprotKB: G3V882
- UniprotKB: Q05028

Rat Pdgfb Predicted Functions

Enables signaling receptor binding activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of cell migration; and response to peptide hormone. Located in several cellular components, including basolateral plasma membrane; dendrite; and neuronal cell body. Used to study asthma; brain infarction; glomerulonephritis; and visual epilepsy. Biomarker of cholestasis; hypertension (multiple); liver cirrhosis; oligohydramnios; and ureteral obstruction. Human ortholog(s) of this gene implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Orthologous to human PDGFB (platelet derived growth factor subunit B).

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