Mouse Prolactin ELISA Kit

$350.00$450.00

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TARGETSPRL
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity5.24 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery73% – 122%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity5.24 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery73% – 122%
Average Recovery0.97
Intra Precision1.5% – 4.2%
Inter-Precision1.7%-4.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ProlactinP monoclonal antibody
Mouse Prolactin freeze-dried standard
Prolactin detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(22×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Prolactin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, Prolactin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Prolactin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PRL

PRL Target Infomation Overview

  • Target Symbol: PRL, prolactin
  • Gene Groups: Growth hormone family; Neuropeptides

PRL, prolactin Target Infomation by Species

HumanMouseRat

Human PRL Target Information

Human PRL Predicted Functions

Predicted to enable hormone activity and prolactin receptor binding activity. Involved in several processes, including negative regulation of endothelial cell proliferation; positive regulation of pri-miRNA transcription by RNA polymerase II; and positive regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in secretory granule. Predicted to be active in extracellular space. Implicated in carotid artery disease. Biomarker of hepatocellular carcinoma; hypoglycemia; obesity; and severe acute respiratory syndrome.

Mouse Prl Target Information

Mouse Prl Predicted Functions

Predicted to enable hormone activity and prolactin receptor binding activity. Acts upstream of or within several processes, including mammary gland development; maternal behavior; and positive regulation of receptor signaling pathway via JAK-STAT. Located in secretory granule. Is expressed in hippocampus; pituitary gland; placenta; and trophoblast giant cell. Human ortholog(s) of this gene implicated in carotid artery disease. Orthologous to human PRL (prolactin).

Rat Prl Target Information

Rat Prl Predicted Functions

Predicted to enable hormone activity and prolactin receptor binding activity. Involved in several processes, including parturition; response to estradiol; and response to lipopolysaccharide. Located in extracellular region and secretory granule. Used to study portal hypertension and prolactinoma. Biomarker of hyperprolactinemia; prolactinoma; and renovascular hypertension. Human ortholog(s) of this gene implicated in carotid artery disease. Orthologous to human PRL (prolactin).

PRL Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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