Mouse RAGE ELISA Kit

$350.00$450.00

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TARGETSAGER
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity7.90 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery97% – 125%
Average Recovery1.05
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity7.90 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery97% – 125%
Average Recovery1.05
Intra Precision2.0% – 3.4%
Inter-Precision2.2% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-RAGE monoclonal antibody
Mouse RAGE freeze-dried standard
RAGE detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(22×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse RAGE antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, RAGE present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of RAGE in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

AGER

AGER Target Infomation Overview

  • Target Symbol: AGER, advanced glycosylation end-product specific receptor
  • Gene Groups: C2-set domain containing; Scavenger receptors
  • Alias: RAGE; SCARJ1; sRAGE
  • Alias Names: receptor for advanced glycation end-products

AGER, advanced glycosylation end-product specific receptor Target Infomation by Species

HumanMouseRat

Human AGER Target Information

  • Target Symbol: AGER, advanced glycosylation end-product specific receptor
  • Alias:
    • advanced glycosylation end product-specific receptor
    • MGC22357
    • RAGE
    • RAGE isoform delta
    • RAGE isoform NtRAGE-delta
    • RAGE isoform sRAGE-delta
    • receptor for advanced glycation end-products variant 20
    • receptor for advanced glycosylation end products
    • receptor for advanced glycosylation end-products
    • SCARJ1
    • sRAGE
  • NCBI_Gene: 177
  • UniProtKB: Q15109

Human AGER Predicted Functions

Enables several functions, including S100 protein binding activity; amyloid-beta binding activity; and identical protein binding activity. Involved in several processes, including modulation of chemical synaptic transmission; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in apical plasma membrane; cell junction; and fibrillar center. Implicated in several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); cystic fibrosis; kidney failure (multiple); and lupus nephritis. Biomarker of several diseases, including artery disease (multiple); chronic kidney disease (multiple); congestive heart failure (multiple); diabetic retinopathy (multiple); and lung disease (multiple).

Mouse Ager Target Information

Mouse Ager Predicted Functions

Enables S100 protein binding activity; advanced glycation end-product binding activity; and heparin binding activity. Involved in several processes, including cellular response to amyloid-beta; negative regulation of long-term synaptic potentiation; and positive regulation of cytokine production. Acts upstream of or within several processes, including induction of positive chemotaxis; negative regulation of advanced glycation end-product receptor activity; and positive regulation of macromolecule metabolic process. Located in extracellular space and plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and lung. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); cystic fibrosis; kidney failure (multiple); and lupus nephritis. Orthologous to human AGER (advanced glycosylation end-product specific receptor).

Rat Ager Target Information

Rat Ager Predicted Functions

Enables S100 protein binding activity and high mobility group box 1 binding activity. Involved in several processes, including positive regulation of apoptotic process; regulation of cell migration; and response to hexose. Located in several cellular components, including basal plasma membrane; cell surface; and neuronal cell body. Used to study several diseases, including diabetic retinopathy; impotence; renal fibrosis; thromboangiitis obliterans; and type 2 diabetes mellitus. Biomarker of several diseases, including bone disease (multiple); cardiovascular system disease (multiple); colorectal carcinoma; diabetic encephalopathy; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); cystic fibrosis; kidney failure (multiple); and lupus nephritis. Orthologous to human AGER (advanced glycosylation end-product specific receptor).

AGER Target News

    7.162
    RAGE inhibition alleviates lipopolysaccharides-induced lung injury via directly suppressing autophagic apoptosis of type II alveolar epithelial cells
    RESPIRATORY RESEARCH

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