EK371

Rat IgG ELISA Kit

$245.00$350.00

Related Target
Speciesrat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.20 ng/mL
Array Range1.56 ng/mL – 100 ng/mL
Assay Time3.5 h
Recovery85% – 110%
Average Recovery100%

ELISA Kit Detail Information

Related Target
Speciesrat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.20 ng/mL
Array Range1.56 ng/mL – 100 ng/mL
Assay Time3.5 h
Recovery85% – 110%
Average Recovery100%
Intra Precision2.3% – 6.7%
Inter Precision3.9% – 6.7%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgG monoclonal antibody
Rat IgG freeze-dried standard
IgG detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IgG antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IgG present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgG in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGG

No description available.

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