Rat IgG2b ELISA Kit

$245.00 – $350.00

Related Target	IGG
Species	rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	1.25 ng/mL
Array Range	7.81 ng/mL – 500 ng/mL
Assay Time	3.5 h
Recovery	85% – 111%
Average Recovery	99%

ELISA Kit Detail Information

Related Target	IGG
Species	rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	1.25 ng/mL
Array Range	7.81 ng/mL – 500 ng/mL
Assay Time	3.5 h
Recovery	85% – 111%
Average Recovery	99%
Intra Precision	6.5% – 7.4%
Inter Precision	3.9% – 9.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgG2b monoclonal antibody Rat IgG2b freeze-dried standard IgG2b detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-rat IgG2b antibodies are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, the IgG2b present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, HRP-labeled detection antibodies are added and incubated. After washing to remove unbound detection antibodies, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of IgG2b in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGG

No description available.

Citations for Rat IgG2b ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.