Rat IgM ELISA Kit

$350.00$450.00

Download:
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.17 ng/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery80% – 110%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.17 ng/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery80% – 110%
Average Recovery0.98
Intra Precision4.8% – 7.7%
Inter-Precision6.1% – 7.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgM monoclonal antibody
Rat IgM freeze-dried standard
IgM detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IgM antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, IgM present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies labeled with horseradish peroxidase (HRP) are added and incubated. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgM in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

4.614
Accumulative Effects of Multifrequency Microwave Exposure with 1.5 GHz and 2.8 GHz on the Structures and Functions of the Immune System
International Journal of Environmental Research and Public Health
10.8
Ginsenoside Rg3 endows liposomes with prolonged blood circulation and reduced accelerated blood clearance
JOURNAL OF CONTROLLED RELEASE
5.195
Myricetin ameliorates experimental autoimmune myocarditis in mice by modulating immune response and inhibiting MCP-1 expression
EUROPEAN JOURNAL OF PHARMACOLOGY
3.1
Sleep deprivation reduced LPS-induced IgG2b production by up-regulating BMAL1 and CLOCK expression
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Related Products

more +

EK1392

$350.00$450.00

EK1341

$350.00$450.00

EK1C01

$350.00$450.00

Same Target