Rat Insulin Competitive ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	INS
SPECIES	Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.15 ng/mL
Range	0.16 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	85% – 116%
Average Recovery	1.03

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.15 ng/mL
Range	0.16 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	85% – 116%
Average Recovery	1.03
Intra Precision	4.58%-7.31%
Inter-Precision	5.88% – 8.85%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Rat Insulin monoclonal antibody Rat Insulin freeze-dried standard Rat Insulin偶联物 Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes a solid-phase competitive enzyme-linked immunosorbent assay (ELISA) technique. Specific monoclonal antibodies against rat insulin are precoated on a high-affinity ELISA plate. Biotinylated insulin and unlabeled insulin or samples are added into the wells of the ELISA plate, competing to bind to the limited binding sites on the coated antibody. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is inversely proportional to the concentration of insulin in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

INS

INS Target Infomation Overview

Target Symbol: INS, insulin
Gene Groups: Neuropeptides
Previous Names: IDDM2; IDDM1
Alias Names: insulin-dependent diabetes mellitus 2

INS, insulin Target Infomation by Species

HumanMouseRat

Human INS Target Information

Target Symbol: INS, insulin
Alias:
- IDDM
- IDDM1
- IDDM2
- ILPR
- insulin-dependent diabetes mellitus 2
- IRDN
- MODY10
- PNDM4
- preproinsulin
- proinsulin
NCBI_Gene: 3630
UniProtKB: P01308

Human INS Predicted Functions

Enables identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of signal transduction; regulation of generation of precursor metabolites and energy; and regulation of protein metabolic process. Located in extracellular space. Implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Biomarker of disease of metabolism (multiple); osteoarthritis; pancreatic ductal carcinoma; and prostate cancer.

Mouse Ins2 Target Information

Target Symbol: Ins2, insulin II
Alias:
- AA986540
- expressed sequence AA986540
- Ins-2
- InsII
- maturity onset diabetes of the young
- maturity onset diabetes of the young 4
- Mody
- Mody4
NCBI_Gene: 16334

Mouse Ins2 Predicted Functions

Predicted to enable identical protein binding activity; protease binding activity; and signaling receptor binding activity. Acts upstream of or within several processes, including cell surface receptor signaling pathway; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; and positive regulation of phosphorylation. Located in cytosol; nucleus; and secretory granule. Part of sno(s)RNA-containing ribonucleoprotein complex. Is expressed in several structures, including brain; extraembryonic component; gut; immune system; and testis. Used to study maturity-onset diabetes of the young; neonatal diabetes; permanent neonatal diabetes mellitus; type 1 diabetes mellitus; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Orthologous to several human genes including INS (insulin).

Rat Ins2 Target Information

Target Symbol: Ins2, insulin 2
Alias:
- C-peptide
- CP-II
- insulin-2
- LOC102549619
- uncharacterized LOC102549619
NCBI_Gene: 24506
UniProtKB: P01323

Rat Ins2 Predicted Functions

Predicted to enable several functions, including identical protein binding activity; signaling receptor binding activity; and zinc ion binding activity. Predicted to be involved in several processes, including negative regulation of catabolic process; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Predicted to act upstream of or within several processes, including cell surface receptor signaling pathway; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; and positive regulation of phosphorylation. Located in extracellular space. Colocalizes with secretory granule. Used to study hypertension. Biomarker of Alzheimer’s disease. Human ortholog(s) of this gene implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Orthologous to human INS (insulin).