Rat Insulin Competitive ELISA Kit

$350.00$450.00

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TARGETSINS
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.15 ng/mL
Range0.16 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery85% – 116%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.15 ng/mL
Range0.16 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery85% – 116%
Average Recovery1.03
Intra Precision4.58%-7.31%
Inter-Precision5.88% – 8.85%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Rat Insulin monoclonal antibody
Rat Insulin freeze-dried standard
Rat Insulin偶联物
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes a solid-phase competitive enzyme-linked immunosorbent assay (ELISA) technique. Specific monoclonal antibodies against rat insulin are precoated on a high-affinity ELISA plate. Biotinylated insulin and unlabeled insulin or samples are added into the wells of the ELISA plate, competing to bind to the limited binding sites on the coated antibody. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is inversely proportional to the concentration of insulin in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

INS

INS Target Infomation Overview

  • Target Symbol: INS, insulin
  • Gene Groups: Neuropeptides
  • Previous Names: IDDM2; IDDM1
  • Alias Names: insulin-dependent diabetes mellitus 2

INS, insulin Target Infomation by Species

HumanMouseRat

Human INS Target Information

Human INS Predicted Functions

Enables identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of signal transduction; regulation of generation of precursor metabolites and energy; and regulation of protein metabolic process. Located in extracellular space. Implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Biomarker of disease of metabolism (multiple); osteoarthritis; pancreatic ductal carcinoma; and prostate cancer.

Mouse Ins2 Target Information

Mouse Ins2 Predicted Functions

Predicted to enable identical protein binding activity; protease binding activity; and signaling receptor binding activity. Acts upstream of or within several processes, including cell surface receptor signaling pathway; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; and positive regulation of phosphorylation. Located in cytosol; nucleus; and secretory granule. Part of sno(s)RNA-containing ribonucleoprotein complex. Is expressed in several structures, including brain; extraembryonic component; gut; immune system; and testis. Used to study maturity-onset diabetes of the young; neonatal diabetes; permanent neonatal diabetes mellitus; type 1 diabetes mellitus; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Orthologous to several human genes including INS (insulin).

Rat Ins2 Target Information

Rat Ins2 Predicted Functions

Predicted to enable several functions, including identical protein binding activity; signaling receptor binding activity; and zinc ion binding activity. Predicted to be involved in several processes, including negative regulation of catabolic process; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Predicted to act upstream of or within several processes, including cell surface receptor signaling pathway; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; and positive regulation of phosphorylation. Located in extracellular space. Colocalizes with secretory granule. Used to study hypertension. Biomarker of Alzheimer’s disease. Human ortholog(s) of this gene implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Orthologous to human INS (insulin).

INS Target News

    6.656
    Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism
    PHYTOMEDICINE
    5.4
    Jianpi Shengqing Huazhuo Formula improves abnormal glucose and lipid metabolism in obesity by regulating mitochondrial biogenesis
    JOURNAL OF ETHNOPHARMACOLOGY
    7.2
    Vitamin D3 alleviates lung fibrosis of type 2 diabetic rats via SIRT3 mediated suppression of pyroptosis
    APOPTOSIS
    32.086
    Controlled Interfacial Polymer Self-assembly Coordinates Ultrahigh Drug Loading and Zero-Order Release in Particles Prepared under Continuous Flow
    ADVANCED MATERIALS
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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