Rat Leptin ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | rat |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL (diluent) |
| Sensitivity | 1.14 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92% – 130% |
| Average Recovery | 107% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | rat |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL (diluent) |
| Sensitivity | 1.14 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92% – 130% |
| Average Recovery | 107% |
| Intra Precision | 3.5% – 4.6% |
| Inter Precision | 4.2% – 4.7% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- Leptin monoclonal antibody Rat Leptin freeze-dried standard Leptin detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat Leptin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Leptin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Leptin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
LEP
LEP Target Infomation Overview
- Target Symbol: LEP, leptin
- Gene Groups: Neuropeptides
- Previous Names: OBS; OB
- Alias Names: leptin (murine obesity homolog); leptin (obesity homolog, mouse)
LEP, leptin Target Infomation by Species
- Human
- Mouse
- Rat
Human LEP Target Information
- Target Symbol: LEP, leptin
- Alias:
- FLJ94114
- LEPD
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
- OB
- obese protein
- obese, mouse, homolog of
- obesity factor
- OBS
- NCBI_Gene: 3952
- UniProtKB: P41159
Human LEP Predicted Functions
Enables leptin receptor binding activity. Involved in several processes, including positive regulation of signal transduction; regulation of cytokine production; and regulation of lymphocyte proliferation. Predicted to be located in cytosol. Predicted to be active in extracellular space. Implicated in several diseases, including alcohol dependence; congenital leptin deficiency; liver disease (multiple); lung disease (multiple); and type 2 diabetes mellitus. Biomarker of several diseases, including alcohol dependence; cystic fibrosis; fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple).
Mouse Lep Target Information
- Target Symbol: Lep, leptin
- Alias:
- ob
- obese
- NCBI_Gene: 16846
Mouse Lep Predicted Functions
Enables DNA binding activity and growth factor activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cytokine production; and positive regulation of signal transduction. Acts upstream of or within several processes, including insulin secretion; monocarboxylic acid metabolic process; and regulation of insulin secretion. Located in cytoplasm and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; central nervous system; early conceptus; and genitourinary system. Used to study abdominal obesity-metabolic syndrome (multiple); fatty liver disease (multiple); obesity; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; liver disease (multiple); lung disease (multiple); and type 2 diabetes mellitus. Orthologous to human LEP (leptin).
Rat Lep Target Information
- Target Symbol: Lep, leptin
- Alias:
- OB
- obese
- obesity (murine homolog leptin)
- obesity factor
- NCBI_Gene: 25608
Rat Lep Predicted Functions
Enables hormone activity and peptide hormone receptor binding activity. Involved in several processes, including positive regulation of cell communication; regulation of apoptotic process; and response to vitamin. Located in cytosol and extracellular space. Used to study several diseases, including alcohol dependence; glucose metabolism disease (multiple); liver disease (multiple); neurodegenerative disease (multiple); and short bowel syndrome. Biomarker of several diseases, including Huntington’s disease; alcohol dependence; fatty liver disease (multiple); lipid metabolism disorder (multiple); and obesity. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human LEP (leptin).
Products with the Same Target - LEP
Citations for Rat Leptin ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK1259
- ELISA Kit
Human Apolipoprotein M/Apo M ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1120
- ELISA Kit
Human Activin A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1317
- ELISA Kit
Human ACE ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1391
- ELISA Kit
Human ACE2 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1223
- ELISA Kit
Human Apolipoprotein D/Apo D ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1275
- ELISA Kit
Human Antithrombin-III/Serpin C1 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.