Rat SCF ELISA Kit

$350.00$450.00

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TARGETSKITLG
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity38.4 pg/mL
Range0.23 ng/mL – 15 ng/mL
Assay Time3.5 h
Recovery81% – 112%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity38.4 pg/mL
Range0.23 ng/mL – 15 ng/mL
Assay Time3.5 h
Recovery81% – 112%
Average Recovery0.93
Intra Precision8.3% – 9.2%
Inter-Precision5.8% – 7.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-SCF monoclonal antibody
Rat SCF freeze-dried standard
Rat SCF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific monoclonal antibodies against rat SCF are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, the SCF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated, followed by washing to remove unbound biotinylated antibodies. Then, HRP-labeled streptavidin is added. After washing, TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of SCF in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

KITLG

KITLG Target Infomation Overview

  • Target Symbol: KITLG, KIT ligand
  • Alias: SCF; SF; Kitl; KL-1; FPH2; SLF; DFNA69
  • Previous Names: MGF
  • Alias Names: mast cell growth factor; stem cell factor; steel factor; familial progressive hyperpigmentation 2

KITLG, KIT ligand Target Infomation by Species

HumanMouseRat

Human KITLG Target Information

  • Target Symbol: KITLG, KIT ligand
  • Alias:
    • c-Kit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • kit-ligand
    • Kitl
    • KL-1
    • mast cell growth factor
    • MGF
    • SCF
    • SF
    • SHEP7
    • SLF
    • steel factor
    • stem cell factor
  • NCBI_Gene: 4254
  • UniProtKB: P21583

Human KITLG Predicted Functions

Predicted to enable cytokine activity and stem cell factor receptor binding activity. Involved in embryonic hemopoiesis; male gonad development; and positive regulation of cell population proliferation. Located in several cellular components, including filopodium; lamellipodium; and plasma membrane. Implicated in autosomal dominant nonsyndromic deafness 69 and familial progressive hyperpigmentation with or without hypopigmentation.

Mouse Kitl Target Information

Mouse Kitl Predicted Functions

Enables cytokine activity and stem cell factor receptor binding activity. Acts upstream of or within several processes, including positive regulation of cell differentiation; positive regulation of cell population proliferation; and positive regulation of protein phosphorylation. Located in extracellular space and plasma membrane. Is integral component of membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in autosomal dominant nonsyndromic deafness 69 and familial progressive hyperpigmentation with or without hypopigmentation. Orthologous to human KITLG (KIT ligand).

Rat Kitlg Target Information

Rat Kitlg Predicted Functions

Predicted to enable cytokine activity and stem cell factor receptor binding activity. Involved in ovarian follicle development and positive regulation of cell population proliferation. Predicted to be located in several cellular components, including extracellular space; filopodium; and lamellipodium. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Human ortholog(s) of this gene implicated in autosomal dominant nonsyndromic deafness 69 and familial progressive hyperpigmentation with or without hypopigmentation. Orthologous to human KITLG (KIT ligand).

KITLG Target News

    Allergic Reaction
    Transplantation
    Tumor Marker

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