Rat Total MMP-9 ELISA Kit

$350.00$450.00

TARGETS MMP9
SPECIES Rat
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL(diluent)
Sensitivity 13.88 pg/mL
Range 78.13 pg/mL – 5000 pg/mL
Assay Time 3.5 h
Recovery 86% – 114%
Average Recovery 1.03
Sample Type Serum, plasma and cell culture supernatant
Sample Volume 100 μL(diluent)
Sensitivity 37.135 pg/mL
Range 78.13 pg/mL – 5000 pg/mL
Assay Time 3.5 h
Recovery 89% – 114%
Average Recovery 0.98
Intra Precision 7.7% – 9.5%
Inter-Precision 5.6% – 8.1%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-9 monoclonal antibody
Rat MMP-9 freeze-dried standard
MMP-9 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat MMP-9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MMP9

MMP9 Target Infomation Overview

  • Target Symbol: MMP9, matrix metallopeptidase 9
  • Gene Groups: M10 matrix metallopeptidases
  • Previous Names: CLG4B
  • Alias Names: matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

MMP9, matrix metallopeptidase 9 Target Infomation by Species

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Human MMP9 Target Information

  • Target Symbol: MMP9, matrix metallopeptidase 9
  • Alias:
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG4B
    • GELB
    • macrophage gelatinase
    • MANDP2
    • matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • matrix metalloproteinase-9
    • MMP-9
    • type V collagenase
  • NCBI_Gene: 4318
  • UniProtKB: P14780

Human MMP9 Predicted Functions

Enables identical protein binding activity; metalloendopeptidase activity; and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of apoptotic signaling pathway; and positive regulation of keratinocyte migration. Located in extracellular space. Implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); brain disease (multiple); carcinoma (multiple); and lung disease (multiple).

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Mouse Mmp9 Target Information

Mouse Mmp9 Predicted Functions

Enables metalloendopeptidase activity. Involved in negative regulation of epithelial cell differentiation involved in kidney development and response to amyloid-beta. Acts upstream of or within several processes, including collagen catabolic process; embryo implantation; and extracellular matrix organization. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; limb mesenchyme; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and lung disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).

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Rat Mmp9 Target Information

Rat Mmp9 Predicted Functions

Enables fibronectin binding activity and metallopeptidase activity. Involved in several processes, including cellular response to cell-matrix adhesion; cellular response to cytokine stimulus; and cellular response to iron(III) ion. Located in extracellular space. Part of protein-containing complex. Used to study artery disease (multiple); brain ischemia (multiple); congestive heart failure; glomerulosclerosis; and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); brain disease (multiple); chronic obstructive pulmonary disease (multiple); glucose metabolism disease (multiple); and kidney disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).

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MMP9 Target News

[catlist tags=”mmp9″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

18.027
Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke
ACS Nano
19.924
Drug-Free Biomimetic Oxygen Supply Nanovehicle Promotes Ischemia-Reperfusion Therapy in Stroke
ADVANCED FUNCTIONAL MATERIALS
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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