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Human ANGPTL4 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 43.6 pg/mL |
| Range | 0.31 pg/mL – 20 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 95% – 119% |
| Average Recovery | 1.09 |
| Intra Precision | 4.8% – 4.9% |
| Inter-Precision | 4.7% – 5.0% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ANGPTL4 monoclonal antibody Human ANGPTL4 freeze-dried standard ANGPTL4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ANGPTL4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, ANGPTL4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ANGPTL4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
ANGPTL4
ANGPTL4 Target Infomation Overview
- Target Symbol: ANGPTL4, angiopoietin like 4
- Gene Groups: Fibrinogen C domain containing; Angiopoietin like family
- Alias: pp1158; PGAR; ARP4; HFARP; FIAF; NL2
- Alias Names: fasting-induced adipose factor; hepatic angiopoietin-related protein; PPARG angiopoietin related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein; angiopoietin-related protein 4; angiopoietin-like 4
ANGPTL4, angiopoietin like 4 Target Infomation by Species
HumanMouseRat
Human ANGPTL4 Target Information
- Target Symbol: ANGPTL4, angiopoietin like 4
- Alias:
- angiopoietin-like 4
- angiopoietin-like protein 4
- angiopoietin-related protein 4
- ANGPTL2
- ARP4
- fasting-induced adipose factor
- FIAF
- HARP
- hepatic angiopoietin-related protein
- hepatic fibrinogen/angiopoietin-related protein
- HFARP
- NL2
- peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein
- PGAR
- pp1158
- PPARG angiopoietin related protein
- TGQTL
- UNQ171
- NCBI_Gene: 51129
- UniProtKB: Q9BY76
Human ANGPTL4 Predicted Functions
Enables identical protein binding activity. Involved in negative regulation of apoptotic process; negative regulation of lipoprotein lipase activity; and protein unfolding. Located in extracellular space.
Mouse Angptl4 Target Information
- Target Symbol: Angptl4, angiopoietin-like 4
- Alias:
- BK89
- FIAF
- HFARP
- NG27
- NCBI_Gene: 57875
Mouse Angptl4 Predicted Functions
Enables enzyme inhibitor activity. Involved in negative regulation of apoptotic process; negative regulation of lipoprotein lipase activity; and triglyceride homeostasis. Acts upstream of or within negative regulation of endothelial cell apoptotic process. Located in extracellular space. Is expressed in several structures, including brain; genitourinary system; hemolymphoid system gland; integumental system; and musculature. Orthologous to human ANGPTL4 (angiopoietin like 4).
Rat Angptl4 Target Information
- Target Symbol: Angptl4, angiopoietin-like 4
- Alias:
- angiopoietin-like protein 4
- angiopoietin-related protein 4
- hepatic fibrinogen/angiopoietin-related protein
- HFARP
- LOC362850
- NCBI_Gene: 362850
- UniProtKB: Q6TMA8
Rat Angptl4 Predicted Functions
Enables identical protein binding activity. Involved in response to hypoxia. Predicted to be located in extracellular region. Predicted to be active in collagen-containing extracellular matrix and extracellular space. Orthologous to human ANGPTL4 (angiopoietin like 4).
