Human Apolipoprotein D/Apo D ELISA Kit

$350.00$450.00

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TARGETSAPOD
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity25.67 pg/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery85% – 103%
Average Recovery0.91
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity25.67 pg/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery85% – 103%
Average Recovery0.91
Intra Precision2.0% – 8.1%
Inter-Precision1.9%-2.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein D/Apo D monoclonal antibody
Human Apolipoprotein D/Apo D freeze-dried standard
Apolipoprotein D/Apo D detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo D antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo D present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo D in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

APOD

APOD Target Infomation Overview

  • Target Symbol: APOD, apolipoprotein D
  • Gene Groups: Apolipoproteins; Lipocalins
  • Alias: Apo-D

APOD, apolipoprotein D Target Infomation by Species

HumanMouseRat

Human APOD Target Information

Human APOD Predicted Functions

Enables cholesterol binding activity. Involved in several processes, including negative regulation of cell-matrix adhesion; negative regulation of macromolecule metabolic process; and negative regulation of protein import into nucleus. Located in extracellular space and perinuclear region of cytoplasm. Implicated in obesity and type 2 diabetes mellitus. Biomarker of Alzheimer’s disease; gestational diabetes; and type 2 diabetes mellitus.

Mouse Apod Target Information

Mouse Apod Predicted Functions

Predicted to enable cholesterol binding activity. Involved in negative regulation of lipoprotein lipid oxidation and response to reactive oxygen species. Predicted to be located in several cellular components, including cytosolic ribosome; neuronal cell body; and perinuclear region of cytoplasm. Predicted to be active in cytoplasm. Is expressed in several structures, including central nervous system; embryo mesenchyme; lip; sensory organ; and skeleton. Human ortholog(s) of this gene implicated in obesity and type 2 diabetes mellitus. Orthologous to human APOD (apolipoprotein D).

Rat Apod Target Information

Rat Apod Predicted Functions

Predicted to enable cholesterol binding activity. Involved in several processes, including aging; nervous system development; and tissue regeneration. Located in several cellular components, including cytosolic ribosome; dendrite; and neuronal cell body. Biomarker of status epilepticus and vasculogenic impotence. Human ortholog(s) of this gene implicated in obesity and type 2 diabetes mellitus. Orthologous to human APOD (apolipoprotein D).

APOD Target News

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