Human BDNF ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	BDNF
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.41 pg/mL
Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	85% – 115%
Average Recovery	1.01

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.41 pg/mL
Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	85% – 115%
Average Recovery	1.01
Intra Precision	5.6% – 6.1%
Inter-Precision	2.9% – 7.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-BDNF monoclonal antibody Human BDNF freeze-dried standard BDNF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BDNF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, BDNF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of BDNF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

BDNF

BDNF Target Infomation Overview

Target Symbol: BDNF, brain derived neurotrophic factor
Gene Groups: Neurotrophins
Alias Names: neurotrophin

BDNF, brain derived neurotrophic factor Target Infomation by Species

HumanMouseRat

Human BDNF Target Information

Target Symbol: BDNF, brain derived neurotrophic factor
Alias:
- abrineurin
- ANON2
- brain-derived neurotrophic factor
- BULN2
- MGC34632
- neurotrophin
NCBI_Gene: 627
UniProtKB: P23560

Human BDNF Predicted Functions

Predicted to enable growth factor activity and nerve growth factor receptor binding activity. Involved in nervous system development; positive regulation of nervous system development; and positive regulation of receptor binding activity. Located in mitochondrion and nuclear speck. Implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Biomarker of several diseases, including autoimmune disease (multiple); lung disease (multiple); mental depression (multiple); neurodegenerative disease (multiple); and open-angle glaucoma (multiple).

Mouse Bdnf Target Information

Target Symbol: Bdnf, brain derived neurotrophic factor
Alias:
- 无
NCBI_Gene: 12064

Mouse Bdnf Predicted Functions

Predicted to enable growth factor activity; nerve growth factor receptor binding activity; and neurotrophin TRKB receptor binding activity. Involved in negative regulation of apoptotic signaling pathway; positive regulation of neuron projection development; and retrograde trans-synaptic signaling by neuropeptide, modulating synaptic transmission. Acts upstream of or within several processes, including generation of neurons; modulation of chemical synaptic transmission; and sensory organ development. Located in several cellular components, including dendrite; perinuclear region of cytoplasm; and secretory granule. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Orthologous to human BDNF (brain derived neurotrophic factor).

Rat Bdnf Target Information

Target Symbol: Bdnf, brain-derived neurotrophic factor
Alias:
- Brain derived neurothrophic factor
- brain derived neurotrophic factor
- MGC105254
NCBI_Gene: 24225

Rat Bdnf Predicted Functions

Enables neurotrophin TRKB receptor binding activity. Involved in several processes, including behavioral response to cocaine; cellular response to norepinephrine stimulus; and regulation of neuronal synaptic plasticity. Acts upstream of or within positive regulation of DNA-binding transcription factor activity; positive regulation of glucocorticoid receptor signaling pathway; and positive regulation of peptidyl-serine phosphorylation. Located in several cellular components, including mitochondrial crista; perikaryon; and synaptic vesicle. Used to study Alzheimer’s disease; cystitis; eye disease (multiple); neuropathy (multiple); and status epilepticus. Biomarker of several diseases, including alcohol use disorder; attention deficit hyperactivity disorder; hypertensive encephalopathy; middle cerebral artery infarction; and sciatic neuropathy. Human ortholog(s) of this gene implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Orthologous to human BDNF (brain derived neurotrophic factor).