Human BMP-2 ELISA Kit

$299.00 – $419.00

DATASHEET

Related Target	BMP2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.73 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	81% – 116%
Average Recovery	96%

SKU: EK1108 Category: ELISA Kit

ELISA Kit Detail Information

Related Target	BMP2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.73 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	81% – 116%
Average Recovery	96%
Intra Precision	2.3% – 3.3%
Inter Precision	1.9% – 5.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-BMP-2 monoclonal antibody Human BMP-2 freeze-dried standard BMP-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BMP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, BMP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of BMP-2 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

BMP2

No description available.

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