Human BMP-7 ELISA Kit

$245.00 – $350.00

Related Target	BMP7
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	2.06 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	74% – 120%
Average Recovery	98%

ELISA Kit Detail Information

Related Target	BMP7
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	2.06 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	74% – 120%
Average Recovery	98%
Intra Precision	4.4% – 5.3%
Inter Precision	2.2% – 4.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TRAIL/TNFSF10 monoclonal antibody Human TRAIL/TNFSF10 freeze-dried standard TRAIL/TNFSF10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BMP-7 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, BMP-7 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of BMP-7 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

BMP7

BMP7 Target Infomation Overview

Target Symbol: BMP7, bone morphogenetic protein 7
Gene Groups: Bone morphogenetic proteins
Alias: OP-1
Alias Names: osteogenic protein 1

BMP7, bone morphogenetic protein 7 Target Infomation by Species

Human
Mouse
Rat

Human BMP7 Target Information

Target Symbol: BMP7, bone morphogenetic protein 7
Alias:
- BMP-7
- OP-1
- osteogenic protein 1
NCBI_Gene: 655
UniProtKB: P18075

Human BMP7 Predicted Functions

Predicted to enable BMP receptor binding activity and cytokine activity. Involved in several processes, including regulation of gene expression; regulation of protein phosphorylation; and transmembrane receptor protein serine/threonine kinase signaling pathway. Acts upstream of or within negative regulation of neuron differentiation and neuron projection morphogenesis. Located in extracellular space. Implicated in prostate cancer. Biomarker of breast cancer; ovarian carcinoma; prostate cancer; and renal cell carcinoma.

Mouse Bmp7 Target Information

Target Symbol: Bmp7, bone morphogenetic protein 7
Alias:
- OP1
- osteogenic protein 1
NCBI_Gene: 12162
- UniprotKB: P23359

Mouse Bmp7 Predicted Functions

Predicted to enable BMP receptor binding activity; cytokine activity; and heparin binding activity. Involved in several processes, including allantois development; animal organ development; and chordate embryonic development. Acts upstream of or within several processes, including embryonic morphogenesis; morphogenesis of a branching epithelium; and regulation of morphogenesis of a branching structure. Located in extracellular space. Is expressed in several structures, including alimentary system; embryo ectoderm; embryo mesenchyme; genitourinary system; and sensory organ. Used to study obstructive sleep apnea and osteoarthritis. Human ortholog(s) of this gene implicated in prostate cancer. Orthologous to human BMP7 (bone morphogenetic protein 7).

Rat Bmp7 Target Information

Target Symbol: Bmp7, bone morphogenetic protein 7
Alias:
- BMP-7
- bone moorphogenic protein-7
NCBI_Gene: 85272
UniProtKB: G3V6W8

Rat Bmp7 Predicted Functions

Enables heparin binding activity. Involved in several processes, including response to estradiol; response to peptide hormone; and response to vitamin D. Located in collagen-containing extracellular matrix; extracellular space; and vesicle. Biomarker of acute kidney tubular necrosis; kidney disease; nephroblastoma; and pyelonephritis. Human ortholog(s) of this gene implicated in prostate cancer. Orthologous to human BMP7 (bone morphogenetic protein 7).

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