Human C-peptide ELISA Kit

$245.00 – $350.00

Related Target	NPPC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.86 pg/mL
Array Range	39.06 pg/mL – 2500 pg/mL
Assay Time	3.5 h
Recovery	75% – 123%
Average Recovery	95%

ELISA Kit Detail Information

Related Target	NPPC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.86 pg/mL
Array Range	39.06 pg/mL – 2500 pg/mL
Assay Time	3.5 h
Recovery	75% – 123%
Average Recovery	95%
Intra Precision	3.6% – 4.5%
Inter Precision	3.8% – 7.4%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-C-peptide monoclonal antibody Human C-peptide freeze-dried standard C-peptide detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human C-Peptide antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, C-Peptide present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of C-Peptide in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

NPPC

NPPC Target Infomation Overview

Target Symbol: NPPC, natriuretic peptide C
Gene Groups: Neuropeptides
Alias: CNP
Alias Names: natriuretic peptide precursor C

NPPC, natriuretic peptide C Target Infomation by Species

Human
Mouse
Rat

Human NPPC Target Information

Target Symbol: NPPC, natriuretic peptide C
Alias:
- C-type natriuretic peptide
- CNP
- CNP2
- natriuretic peptide precursor C
- natriuretic peptide precursor type C
NCBI_Gene: 4880
UniProtKB: P23582

Human NPPC Predicted Functions

Enables hormone receptor binding activity. Involved in protein folding and receptor guanylyl cyclase signaling pathway. Acts upstream of or within negative regulation of meiotic cell cycle and negative regulation of oocyte maturation. Part of protein-containing complex. Implicated in hypertension. Biomarker of arteriosclerosis; end stage renal disease; hypertension; and toxic shock syndrome.

Mouse Nppc Target Information

Target Symbol: Nppc, natriuretic peptide type C
Alias:
- CNP
- lbab
- long bone abnormality
- natriuretic peptide precursor C
NCBI_Gene: 18159
- UniprotKB: Q61839
- UniprotKB: Q544K5

Mouse Nppc Predicted Functions

Enables signaling receptor binding activity. Involved in growth plate cartilage chondrocyte differentiation and growth plate cartilage chondrocyte proliferation. Acts upstream of or within several processes, including negative regulation of meiotic cell cycle; negative regulation of oocyte maturation; and regulation of growth. Predicted to be located in extracellular space and secretory granule. Predicted to be part of protein-containing complex. Is expressed in central nervous system and vertebral cartilage condensation. Human ortholog(s) of this gene implicated in hypertension. Orthologous to human NPPC (natriuretic peptide C).

Rat Nppc Target Information

Target Symbol: Nppc, natriuretic peptide C
Alias:
- C-type natriuretic peptide
- natriuretic peptide precursor C
- natriuretic peptide precursor type C
NCBI_Gene: 114593
UniProtKB: P55207

Rat Nppc Predicted Functions

Enables hormone activity. Involved in several processes, including negative regulation of biosynthetic process; positive regulation of cGMP-mediated signaling; and positive regulation of osteoblast differentiation. Acts upstream of or within receptor guanylyl cyclase signaling pathway. Located in extracellular space and secretory granule. Biomarker of aortic valve stenosis and heart disease. Human ortholog(s) of this gene implicated in hypertension. Orthologous to human NPPC (natriuretic peptide C).

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