Human Carbonic Anhydrase XII/CA12 ELISA Kit

$350.00$450.00

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TARGETSCA12
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity1.72 pg/mL
Range78.13 ng/mL – 5000 ng/mL
Assay Time3.5 h
Recovery79% – 109%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity1.72 pg/mL
Range78.13 ng/mL – 5000 ng/mL
Assay Time3.5 h
Recovery79% – 109%
Average Recovery0.93
Intra Precision2.1% – 2.3%
Inter-Precision2.1%-3.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Carbonic Anhydrase XII/ CA12 monoclonal antibody
Human Carbonic Anhydrase XII/ CA12 freeze-dried standard
Human Carbonic Anhydrase XII/ CA12 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CA12 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CA12 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CA12 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CA12

CA12 Target Infomation Overview

  • Target Symbol: CA12, carbonic anhydrase 12
  • Gene Groups: Carbonic anhydrases
  • Alias: HsT18816
  • Alias Names: carbonic anhydrase XII

CA12, carbonic anhydrase 12 Target Infomation by Species

HumanMouseRat

Human CA12 Target Information

  • Target Symbol: CA12, carbonic anhydrase 12
  • Alias:
    • CA-XII
    • carbonate dehydratase XII
    • carbonic anhydrase XII
    • carbonic dehydratase
    • CAXII
    • FLJ20151
    • HsT18816
    • T18816
    • tumor antigen HOM-RCC-3.1.3
  • NCBI_Gene: 771
  • UniProtKB: O43570

Human CA12 Predicted Functions

Predicted to enable carbonate dehydratase activity. Involved in chloride ion homeostasis. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Implicated in isolated hyperchlorhidrosis.

Mouse Car12 Target Information

Mouse Car12 Predicted Functions

Enables carbonate dehydratase activity. Predicted to be involved in chloride ion homeostasis and one-carbon metabolic process. Predicted to act upstream of or within carbon dioxide transport and regulation of pH. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in isolated hyperchlorhidrosis. Orthologous to human CA12 (carbonic anhydrase 12).

Rat Ca12 Target Information

Rat Ca12 Predicted Functions

Predicted to enable carbonate dehydratase activity. Predicted to be involved in chloride ion homeostasis and one-carbon metabolic process. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Human ortholog(s) of this gene implicated in isolated hyperchlorhidrosis. Orthologous to human CA12 (carbonic anhydrase 12).

CA12 Target News

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