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Human Carbonic Anhydrase XII/CA12 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 1.72 pg/mL |
| Range | 78.13 ng/mL – 5000 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 79% – 109% |
| Average Recovery | 0.93 |
| Intra Precision | 2.1% – 2.3% |
| Inter-Precision | 2.1%-3.7% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Carbonic Anhydrase XII/ CA12 monoclonal antibody Human Carbonic Anhydrase XII/ CA12 freeze-dried standard Human Carbonic Anhydrase XII/ CA12 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CA12 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CA12 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CA12 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CA12
CA12 Target Infomation Overview
- Target Symbol: CA12, carbonic anhydrase 12
- Gene Groups: Carbonic anhydrases
- Alias: HsT18816
- Alias Names: carbonic anhydrase XII
CA12, carbonic anhydrase 12 Target Infomation by Species
HumanMouseRat
Human CA12 Target Information
- Target Symbol: CA12, carbonic anhydrase 12
- Alias:
- CA-XII
- carbonate dehydratase XII
- carbonic anhydrase XII
- carbonic dehydratase
- CAXII
- FLJ20151
- HsT18816
- T18816
- tumor antigen HOM-RCC-3.1.3
- NCBI_Gene: 771
- UniProtKB: O43570
Human CA12 Predicted Functions
Predicted to enable carbonate dehydratase activity. Involved in chloride ion homeostasis. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Implicated in isolated hyperchlorhidrosis.
Mouse Car12 Target Information
- Target Symbol: Car12, carbonic anhydrase 12
- Alias:
- 2310047E01Rik
- AI314958
- CA XII
- expressed sequence AI314958
- RIKEN cDNA 2310047E01 gene
- NCBI_Gene: 76459
Mouse Car12 Predicted Functions
Enables carbonate dehydratase activity. Predicted to be involved in chloride ion homeostasis and one-carbon metabolic process. Predicted to act upstream of or within carbon dioxide transport and regulation of pH. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in isolated hyperchlorhidrosis. Orthologous to human CA12 (carbonic anhydrase 12).
Rat Ca12 Target Information
- Target Symbol: Ca12, carbonic anhydrase 12
- Alias:
- Car12
- carbonic anyhydrase 12
- LOC363085
- membrane-bound carbonic anhydrase 12
- NCBI_Gene: 363085
- UniProtKB: A2IBE2
Rat Ca12 Predicted Functions
Predicted to enable carbonate dehydratase activity. Predicted to be involved in chloride ion homeostasis and one-carbon metabolic process. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Human ortholog(s) of this gene implicated in isolated hyperchlorhidrosis. Orthologous to human CA12 (carbonic anhydrase 12).
