Name: Human Carbonic Anhydrase XII/CA12 ELISA Kit
SKU: EK1245
Availability: InStock

EK1245

Human Carbonic Anhydrase XII/CA12 ELISA Kit

$245.00 – $350.00

Related Target	CA12
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	1.72 pg/mL
Array Range	78.13 ng/mL – 5000 ng/mL
Assay Time	3.5 h
Recovery	79% – 109%
Average Recovery	93%

Size

Clear

ELISA Kit Detail Information

Related Target	CA12
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	1.72 pg/mL
Array Range	78.13 ng/mL – 5000 ng/mL
Assay Time	3.5 h
Recovery	79% – 109%
Average Recovery	93%
Intra Precision	2.1% – 2.3%
Inter Precision	2.1% - 3.7%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Carbonic Anhydrase XII/ CA12 monoclonal antibody Human Carbonic Anhydrase XII/ CA12 freeze-dried standard Human Carbonic Anhydrase XII/ CA12 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CA12 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CA12 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CA12 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CA12

CA12 Target Infomation Overview

Target Symbol: CA12, carbonic anhydrase 12
Gene Groups: Carbonic anhydrases
Alias: HsT18816
Alias Names: carbonic anhydrase XII

CA12, carbonic anhydrase 12 Target Infomation by Species

Human
Mouse
Rat

Human CA12 Target Information

Target Symbol: CA12, carbonic anhydrase 12
Alias:
- CA-XII
- carbonate dehydratase XII
- carbonic anhydrase XII
- carbonic dehydratase
- CAXII
- FLJ20151
- HsT18816
- T18816
- tumor antigen HOM-RCC-3.1.3
NCBI_Gene: 771
UniProtKB: O43570

Human CA12 Predicted Functions

Predicted to enable carbonate dehydratase activity. Involved in chloride ion homeostasis. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Implicated in isolated hyperchlorhidrosis.

Mouse Car12 Target Information

Target Symbol: Car12, carbonic anhydrase 12
Alias:
- 2310047E01Rik
- AI314958
- CA XII
- expressed sequence AI314958
- RIKEN cDNA 2310047E01 gene
NCBI_Gene: 76459

Mouse Car12 Predicted Functions

Enables carbonate dehydratase activity. Predicted to be involved in chloride ion homeostasis and one-carbon metabolic process. Predicted to act upstream of or within carbon dioxide transport and regulation of pH. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in isolated hyperchlorhidrosis. Orthologous to human CA12 (carbonic anhydrase 12).

Rat Ca12 Target Information

Target Symbol: Ca12, carbonic anhydrase 12
Alias:
- Car12
- carbonic anyhydrase 12
- LOC363085
- membrane-bound carbonic anhydrase 12
NCBI_Gene: 363085
UniProtKB: A2IBE2

Rat Ca12 Predicted Functions

Predicted to enable carbonate dehydratase activity. Predicted to be involved in chloride ion homeostasis and one-carbon metabolic process. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Human ortholog(s) of this gene implicated in isolated hyperchlorhidrosis. Orthologous to human CA12 (carbonic anhydrase 12).

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