Human Carbonic Anhydrase XIV/ CA14 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CA14
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL 1×Assay Buffer and 50 μL sample
Sensitivity	6.91 pg/mL
Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	70% – 129%
Average Recovery	0.94

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL 1×Assay Buffer and 50 μL sample
Sensitivity	6.91 pg/mL
Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	70% – 129%
Average Recovery	0.94
Intra Precision	2.5% – 5.4%
Inter-Precision	3.9%-4.5%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Carbonic Anhydrase XIV/ CA14 monoclonal antibody Human Carbonic Anhydrase XIV/ CA14 freeze-dried standard Carbonic Anhydrase XIV/ CA14 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CA14 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CA14 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CA14 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CA14

CA14 Target Infomation Overview

Target Symbol: CA14, carbonic anhydrase 14
Gene Groups: Carbonic anhydrases
Alias Names: carbonic anhydrase XIV

CA14, carbonic anhydrase 14 Target Infomation by Species

HumanMouseRat

Human CA14 Target Information

Target Symbol: CA14, carbonic anhydrase 14
Alias:
- CA-XIV
- carbonate dehydratase XIV
- carbonic anhydrase XIV
- carbonic dehydratase
- CAXiV
NCBI_Gene: 23632
UniProtKB: Q9ULX7

Human CA14 Predicted Functions

Predicted to enable carbonate dehydratase activity. Predicted to be involved in one-carbon metabolic process. Predicted to be integral component of membrane. Predicted to be active in plasma membrane.

Mouse Car14 Target Information

Target Symbol: Car14, carbonic anhydrase 14
Alias:
- AW536446
- CA XIV
- expressed sequence AW536446
NCBI_Gene: 23831

Mouse Car14 Predicted Functions

Enables carbonate dehydratase activity. Predicted to be involved in one-carbon metabolic process. Predicted to act upstream of or within carbon dioxide transport and regulation of pH. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including central nervous system; cranium; epiblast; eye; and urinary system. Orthologous to human CA14 (carbonic anhydrase 14).