Human Carbonic Anhydrase XIV/ CA14 ELISA Kit

$350.00$450.00

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TARGETSCA14
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL 1×Assay Buffer and 50 μL sample
Sensitivity6.91 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery70% – 129%
Average Recovery0.94
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL 1×Assay Buffer and 50 μL sample
Sensitivity6.91 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery70% – 129%
Average Recovery0.94
Intra Precision2.5% – 5.4%
Inter-Precision3.9%-4.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Carbonic Anhydrase XIV/ CA14 monoclonal antibody
Human Carbonic Anhydrase XIV/ CA14 freeze-dried standard
Carbonic Anhydrase XIV/ CA14 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CA14 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CA14 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CA14 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CA14

CA14 Target Infomation Overview

  • Target Symbol: CA14, carbonic anhydrase 14
  • Gene Groups: Carbonic anhydrases
  • Alias Names: carbonic anhydrase XIV

CA14, carbonic anhydrase 14 Target Infomation by Species

HumanMouseRat

Human CA14 Target Information

Human CA14 Predicted Functions

Predicted to enable carbonate dehydratase activity. Predicted to be involved in one-carbon metabolic process. Predicted to be integral component of membrane. Predicted to be active in plasma membrane.

Mouse Car14 Target Information

Mouse Car14 Predicted Functions

Enables carbonate dehydratase activity. Predicted to be involved in one-carbon metabolic process. Predicted to act upstream of or within carbon dioxide transport and regulation of pH. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including central nervous system; cranium; epiblast; eye; and urinary system. Orthologous to human CA14 (carbonic anhydrase 14).

Rat Ca14 Target Information

Rat Ca14 Predicted Functions

Predicted to enable carbonate dehydratase activity. Predicted to be involved in one-carbon metabolic process. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Orthologous to human CA14 (carbonic anhydrase 14).

CA14 Target News

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