Human CCL17/TARC ELISA Kit

$245.00 – $350.00

Related Target	CCL17
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.33 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	91% – 116%
Average Recovery	101%

ELISA Kit Detail Information

Related Target	CCL17
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.33 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	91% – 116%
Average Recovery	101%
Intra Precision	1.8% – 3.9%
Inter Precision	1.2% – 3.5%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL17/TARCA monoclonal antibody Human CCL17/TARCA freeze-dried standard CCL17/TARCA detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL17 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL17 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL17 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CCL17

CCL17 Target Infomation Overview

Target Symbol: CCL17, C-C motif chemokine ligand 17
Gene Groups: Chemokine ligands
Alias: TARC; ABCD-2
Previous Names: SCYA17
Alias Names: small inducible cytokine subfamily A (Cys-Cys), member 17; chemokine (C-C motif) ligand 17

CCL17, C-C motif chemokine ligand 17 Target Infomation by Species

Human
Mouse
Rat

Human CCL17 Target Information

Target Symbol: CCL17, C-C motif chemokine ligand 17
Alias:
- A-152E5.3
- ABCD-2
- C-C motif chemokine 17
- CC chemokine TARC
- chemokine (C-C motif) ligand 17
- MGC138271
- MGC138273
- SCYA17
- small inducible cytokine subfamily A (Cys-Cys), member 17
- small-inducible cytokine A17
- T cell-directed CC chemokine
- TARC
- thymus and activation-regulated chemokine
NCBI_Gene: 6361
UniProtKB: Q92583

Human CCL17 Predicted Functions

Predicted to enable CCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including allergic bronchopulmonary aspergillosis; cystic fibrosis; lung disease (multiple); pulmonary eosinophilia; and respiratory syncytial virus infectious disease.

Mouse Ccl17 Target Information

Target Symbol: Ccl17, chemokine (C-C motif) ligand 17
Alias:
- ABCD-2
- Scya17
- small inducible cytokine subfamily A17
- TARC
NCBI_Gene: 20295

Mouse Ccl17 Predicted Functions

Predicted to enable CCR4 chemokine receptor binding activity and chemokine activity. Acts upstream of or within negative regulation of myoblast differentiation. Predicted to be active in extracellular space. Is expressed in Sertoli cell; central nervous system; hemolymphoid system; and retina. Orthologous to human CCL17 (C-C motif chemokine ligand 17).

Rat Ccl17 Target Information

Target Symbol: Ccl17, C-C motif chemokine ligand 17
Alias:
- C-C motif chemokine 17
- chemokine (C-C motif) ligand 17
- Scya17
- small inducible cytokine subfamily A (Cys-Cys), member 17
- Tarc
NCBI_Gene: 117518
- UniprotKB: E9PTY9
- UniprotKB: Q9ERE0

Rat Ccl17 Predicted Functions

Enables CCR4 chemokine receptor binding activity. Predicted to be involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and positive regulation of ERK1 and ERK2 cascade. Predicted to act upstream of or within negative regulation of myoblast differentiation. Predicted to be active in extracellular space. Biomarker of anti-basement membrane glomerulonephritis; asthma; pneumonia; and pulmonary fibrosis. Orthologous to human CCL17 (C-C motif chemokine ligand 17).

Citations for Human CCL17/TARC ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.