Human CCL25/TECK ELISA Kit

$245.00 – $350.00

Related Target	CCL25
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.06 pg/mL
Array Range	31.3 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	72% – 94%
Average Recovery	86%

ELISA Kit Detail Information

Related Target	CCL25
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.06 pg/mL
Array Range	31.3 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	72% – 94%
Average Recovery	86%
Intra Precision	1.0% – 3.9%
Inter Precision	1.1% - 4.7%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL25 monoclonal antibody Human CCL25 freeze-dried standard CCL25 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL25 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the CCL25 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL25 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CCL25

CCL25 Target Infomation Overview

Target Symbol: CCL25, C-C motif chemokine ligand 25
Gene Groups: Chemokine ligands
Alias: TECK; Ckb15
Previous Names: SCYA25
Alias Names: Ck beta-15; thymus expressed chemokine; TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; chemokine (C-C motif) ligand 25

CCL25, C-C motif chemokine ligand 25 Target Infomation by Species

Human
Mouse
Rat

Human CCL25 Target Information

Target Symbol: CCL25, C-C motif chemokine ligand 25
Alias:
- C-C motif chemokine 25
- chemokine (C-C motif) ligand 25
- chemokine TECK
- Ck beta-15
- Ckb15
- MGC150327
- SCYA25
- small inducible cytokine subfamily A (Cys-Cys), member 25
- small-inducible cytokine A25
- TECK
- TECKvar
- thymus expressed chemokine
- thymus-expressed chemokine
NCBI_Gene: 6370
UniProtKB: O15444

Human CCL25 Predicted Functions

Enables CCR10 chemokine receptor binding activity and chemokine activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; negative regulation of leukocyte tethering or rolling; and positive regulation of cell-matrix adhesion. Predicted to be located in extracellular region. Predicted to be active in extracellular space.

Mouse Ccl25 Target Information

Target Symbol: Ccl25, chemokine (C-C motif) ligand 25
Alias:
- AI852536
- CKb15
- expressed sequence AI852536
- Scya25
- small inducible cytokine A25
- TECK
NCBI_Gene: 20300

Mouse Ccl25 Predicted Functions

Enables chemokine activity. Acts upstream of or within chemotaxis and leukocyte migration. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in 3rd branchial pouch; central nervous system; hemolymphoid system; retina; and thymus/parathyroid primordium. Orthologous to human CCL25 (C-C motif chemokine ligand 25).

Rat Ccl25 Target Information

Target Symbol: Ccl25, C-C motif chemokine ligand 25
Alias:
- C-C motif chemokine 25
- chemokine (C-C motif) ligand 25
- LOC360750
- MGC125074
NCBI_Gene: 360750
UniProtKB: Q32PX4

Rat Ccl25 Predicted Functions

Predicted to enable CCR10 chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and negative regulation of leukocyte tethering or rolling. Predicted to act upstream of or within chemotaxis and leukocyte migration. Predicted to be active in extracellular space. Orthologous to human CCL25 (C-C motif chemokine ligand 25).

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