Human CD258/LIGHT/TNFSF14 ELISA Kit

$350.00$450.00

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TARGETSTNFSF14
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity22.26 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery80% – 118%
Average Recovery0.91
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity22.26 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery80% – 118%
Average Recovery0.91
Intra Precision3.6% – 4.8%
Inter-Precision2.6% – 4.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CD258/LIGHT/TNFSF14 monoclonal antibody
Human CD258/LIGHT/TNFSF14 freeze-dried standard
CD258/LIGHT/TNFSF14 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD258 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CD258 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD258 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNFSF14

TNFSF14 Target Infomation Overview

  • Target Symbol: TNFSF14, TNF superfamily member 14
  • Gene Groups: CD molecules; Tumor necrosis factor superfamily
  • Alias: LIGHT; LTg; HVEM-L; CD258
  • Alias Names: tumor necrosis factor (ligand) superfamily, member 14

TNFSF14, TNF superfamily member 14 Target Infomation by Species

HumanMouseRat

Human TNFSF14 Target Information

  • Target Symbol: TNFSF14, TNF superfamily member 14
  • Alias:
    • CD258
    • delta transmembrane LIGHT
    • herpes virus entry mediator ligand
    • herpesvirus entry mediator A
    • herpesvirus entry mediator ligand
    • herpesvirus entry mediator-ligand
    • HVEML
    • ligand for herpesvirus entry mediator
    • LIGHT
    • LTg
    • TNLG1D
    • TR2
    • tumor necrosis factor (ligand) superfamily member 14
    • tumor necrosis factor (ligand) superfamily, member 14
    • tumor necrosis factor ligand 1D
    • tumor necrosis factor ligand superfamily member 14
    • tumor necrosis factor receptor-like 2
    • tumor necrosis factor superfamily member 14
    • tumor necrosis factor superfamily member LIGHT
  • NCBI_Gene: 8740
  • UniProtKB: O43557

Human TNFSF14 Predicted Functions

Enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process; identical protein binding activity; and signaling receptor binding activity. Involved in T cell costimulation; cellular response to mechanical stimulus; and positive regulation of NIK/NF-kappaB signaling. Predicted to be located in plasma membrane.

Mouse Tnfsf14 Target Information

Mouse Tnfsf14 Predicted Functions

Predicted to enable cysteine-type endopeptidase inhibitor activity involved in apoptotic process; identical protein binding activity; and signaling receptor binding activity. Acts upstream of or within several processes, including T cell costimulation; positive regulation of T cell chemotaxis; and positive regulation of myoblast fusion. Predicted to be located in plasma membrane. Used to study IgA glomerulonephritis. Orthologous to human TNFSF14 (TNF superfamily member 14).

Rat Tnfsf14 Target Information

  • Target Symbol: Tnfsf14, TNF superfamily member 14
  • Alias:
    • LOC301133
    • similar to Tumor necrosis factor ligand superfamily member 14 (CD258 antigen)
    • Tnlg1d
    • tumor necrosis factor (ligand) superfamily, member 14
    • tumor necrosis factor ligand 1d
    • tumor necrosis factor ligand superfamily member 14
    • tumor necrosis factor superfamily member 14
  • NCBI_Gene: 301133
  • UniProtKB: M0RCN2

Rat Tnfsf14 Predicted Functions

Predicted to enable cysteine-type endopeptidase inhibitor activity involved in apoptotic process; identical protein binding activity; and signaling receptor binding activity. Predicted to be involved in cellular response to mechanical stimulus and positive regulation of NIK/NF-kappaB signaling. Predicted to act upstream of or within several processes, including T cell costimulation; positive regulation of T cell chemotaxis; and positive regulation of myoblast fusion. Predicted to be integral component of membrane. Orthologous to human TNFSF14 (TNF superfamily member 14).

TNFSF14 Target News

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