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Human CD258/LIGHT/TNFSF14 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 80 μL 1×Assay Buffer and 20 μL sample |
| Sensitivity | 22.26 pg/mL |
| Range | 0.31 ng/mL – 20 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 118% |
| Average Recovery | 0.91 |
| Intra Precision | 3.6% – 4.8% |
| Inter-Precision | 2.6% – 4.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CD258/LIGHT/TNFSF14 monoclonal antibody Human CD258/LIGHT/TNFSF14 freeze-dried standard CD258/LIGHT/TNFSF14 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD258 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CD258 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD258 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TNFSF14
TNFSF14 Target Infomation Overview
- Target Symbol: TNFSF14, TNF superfamily member 14
- Gene Groups: CD molecules; Tumor necrosis factor superfamily
- Alias: LIGHT; LTg; HVEM-L; CD258
- Alias Names: tumor necrosis factor (ligand) superfamily, member 14
TNFSF14, TNF superfamily member 14 Target Infomation by Species
HumanMouseRat
Human TNFSF14 Target Information
- Target Symbol: TNFSF14, TNF superfamily member 14
- Alias:
- CD258
- delta transmembrane LIGHT
- herpes virus entry mediator ligand
- herpesvirus entry mediator A
- herpesvirus entry mediator ligand
- herpesvirus entry mediator-ligand
- HVEML
- ligand for herpesvirus entry mediator
- LIGHT
- LTg
- TNLG1D
- TR2
- tumor necrosis factor (ligand) superfamily member 14
- tumor necrosis factor (ligand) superfamily, member 14
- tumor necrosis factor ligand 1D
- tumor necrosis factor ligand superfamily member 14
- tumor necrosis factor receptor-like 2
- tumor necrosis factor superfamily member 14
- tumor necrosis factor superfamily member LIGHT
- NCBI_Gene: 8740
- UniProtKB: O43557
Human TNFSF14 Predicted Functions
Enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process; identical protein binding activity; and signaling receptor binding activity. Involved in T cell costimulation; cellular response to mechanical stimulus; and positive regulation of NIK/NF-kappaB signaling. Predicted to be located in plasma membrane.
Mouse Tnfsf14 Target Information
- Target Symbol: Tnfsf14, tumor necrosis factor (ligand) superfamily, member 14
- Alias:
- HVEM-L
- LIGHT
- NCBI_Gene: 50930
Mouse Tnfsf14 Predicted Functions
Predicted to enable cysteine-type endopeptidase inhibitor activity involved in apoptotic process; identical protein binding activity; and signaling receptor binding activity. Acts upstream of or within several processes, including T cell costimulation; positive regulation of T cell chemotaxis; and positive regulation of myoblast fusion. Predicted to be located in plasma membrane. Used to study IgA glomerulonephritis. Orthologous to human TNFSF14 (TNF superfamily member 14).
Rat Tnfsf14 Target Information
- Target Symbol: Tnfsf14, TNF superfamily member 14
- Alias:
- LOC301133
- similar to Tumor necrosis factor ligand superfamily member 14 (CD258 antigen)
- Tnlg1d
- tumor necrosis factor (ligand) superfamily, member 14
- tumor necrosis factor ligand 1d
- tumor necrosis factor ligand superfamily member 14
- tumor necrosis factor superfamily member 14
- NCBI_Gene: 301133
- UniProtKB: M0RCN2
Rat Tnfsf14 Predicted Functions
Predicted to enable cysteine-type endopeptidase inhibitor activity involved in apoptotic process; identical protein binding activity; and signaling receptor binding activity. Predicted to be involved in cellular response to mechanical stimulus and positive regulation of NIK/NF-kappaB signaling. Predicted to act upstream of or within several processes, including T cell costimulation; positive regulation of T cell chemotaxis; and positive regulation of myoblast fusion. Predicted to be integral component of membrane. Orthologous to human TNFSF14 (TNF superfamily member 14).
