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Human CXCL12/SDF-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 18.13 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 89% – 110% |
| Average Recovery | 1.02 |
| Intra Precision | 2.4% – 2.6% |
| Inter-Precision | 3.0% – 4.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL12/SDF-1 monoclonal antibody Human CXCL12/SDF-1 freeze-dried standard CXCL12/SDF-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL12 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL12 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL12 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CXCL12
CXCL12 Target Infomation Overview
- Target Symbol: CXCL12, C-X-C motif chemokine ligand 12
- Gene Groups: Chemokine ligands
- Alias: SCYB12; SDF-1a; SDF-1b; PBSF; TLSF-a; TLSF-b; TPAR1
- Previous Names: SDF1A; SDF1B; SDF1
- Alias Names: stromal cell-derived factor 1; chemokine (C-X-C motif) ligand 12
CXCL12, C-X-C motif chemokine ligand 12 Target Infomation by Species
HumanMouseRat
Human CXCL12 Target Information
- Target Symbol: CXCL12, C-X-C motif chemokine ligand 12
- Alias:
- chemokine (C-X-C motif) ligand 12
- intercrine reduced in hepatomas
- IRH
- PBSF
- pre-B cell growth-stimulating factor
- SCYB12
- SDF-1a
- SDF-1b
- SDF1
- SDF1A
- SDF1B
- stromal cell-derived factor 1
- TLSF
- TLSF-a
- TLSF-b
- TPAR1
- NCBI_Gene: 6387
- UniProtKB: P48061
Human CXCL12 Predicted Functions
Enables CXCR chemokine receptor binding activity; chemokine activity; and integrin binding activity. Involved in several processes, including integrin activation; negative regulation of apoptotic process; and regulation of leukocyte migration. Acts upstream of or within positive regulation of T cell migration and positive regulation of cell adhesion. Located in extracellular exosome. Part of collagen-containing extracellular matrix. Implicated in breast carcinoma; diabetic angiopathy; human immunodeficiency virus infectious disease; and type 1 diabetes mellitus. Biomarker of diabetic retinopathy; insulinoma; periodontitis; and type 2 diabetes mellitus.
Mouse Cxcl12 Target Information
- Target Symbol: Cxcl12, chemokine (C-X-C motif) ligand 12
- Alias:
- AI174028
- expressed sequence AI174028
- PBSF
- PBSF/SDF-1
- Scyb12
- SDF-1
- Sdf1
- Sdf1a
- Sdf1b
- stromal cell derived factor 1
- stromal cell derived factor 1A
- stromal cell derived factor 1B
- TLSF-a
- TLSF-b
- TPAR1
- NCBI_Gene: 20315
Mouse Cxcl12 Predicted Functions
Enables chemokine activity. Involved in dopaminergic neuron differentiation. Acts upstream of or within several processes, including gamete generation; nervous system development; and positive regulation of intracellular signal transduction. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in breast carcinoma; diabetic angiopathy; human immunodeficiency virus infectious disease; and type 1 diabetes mellitus. Orthologous to human CXCL12 (C-X-C motif chemokine ligand 12).
Rat Cxcl12 Target Information
- Target Symbol: Cxcl12, C-X-C motif chemokine ligand 12
- Alias:
- chemokine (C-X-C motif) ligand 12
- chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
- SDF-1 gamma
- Sdf1
- Stromal cell-derived factor 1
- stromal cell-derived factor-1 gamma
- NCBI_Gene: 24772
Rat Cxcl12 Predicted Functions
Predicted to enable CXCR chemokine receptor binding activity; chemokine activity; and integrin binding activity. Involved in several processes, including detection of stimulus involved in sensory perception of pain; nervous system development; and regulation of ion transport. Located in several cellular components, including extracellular space; nucleus; and plasma membrane. Used to study myocardial infarction. Biomarker of anti-basement membrane glomerulonephritis; brain ischemia; myocardial infarction; status epilepticus; and transient cerebral ischemia. Human ortholog(s) of this gene implicated in breast carcinoma; diabetic angiopathy; human immunodeficiency virus infectious disease; and type 1 diabetes mellitus. Orthologous to human CXCL12 (C-X-C motif chemokine ligand 12).
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