Human CXCL12/SDF-1 ELISA Kit

$245.00 – $350.00

Related Target	CXCL12
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	18.13 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	89% – 110%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	CXCL12
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	18.13 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	89% – 110%
Average Recovery	102%
Intra Precision	2.4% – 2.6%
Inter Precision	3.0% – 4.5%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL12/SDF-1 monoclonal antibody Human CXCL12/SDF-1 freeze-dried standard CXCL12/SDF-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL12 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL12 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL12 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CXCL12

CXCL12 Target Infomation Overview

Target Symbol: CXCL12, C-X-C motif chemokine ligand 12
Gene Groups: Chemokine ligands
Alias: SCYB12; SDF-1a; SDF-1b; PBSF; TLSF-a; TLSF-b; TPAR1
Previous Names: SDF1A; SDF1B; SDF1
Alias Names: stromal cell-derived factor 1; chemokine (C-X-C motif) ligand 12

CXCL12, C-X-C motif chemokine ligand 12 Target Infomation by Species

Human
Mouse
Rat

Human CXCL12 Target Information

Target Symbol: CXCL12, C-X-C motif chemokine ligand 12
Alias:
- chemokine (C-X-C motif) ligand 12
- intercrine reduced in hepatomas
- IRH
- PBSF
- pre-B cell growth-stimulating factor
- SCYB12
- SDF-1a
- SDF-1b
- SDF1
- SDF1A
- SDF1B
- stromal cell-derived factor 1
- TLSF
- TLSF-a
- TLSF-b
- TPAR1
NCBI_Gene: 6387
UniProtKB: P48061

Human CXCL12 Predicted Functions

Enables CXCR chemokine receptor binding activity; chemokine activity; and integrin binding activity. Involved in several processes, including integrin activation; negative regulation of apoptotic process; and regulation of leukocyte migration. Acts upstream of or within positive regulation of T cell migration and positive regulation of cell adhesion. Located in extracellular exosome. Part of collagen-containing extracellular matrix. Implicated in breast carcinoma; diabetic angiopathy; human immunodeficiency virus infectious disease; and type 1 diabetes mellitus. Biomarker of diabetic retinopathy; insulinoma; periodontitis; and type 2 diabetes mellitus.

Mouse Cxcl12 Target Information

Target Symbol: Cxcl12, chemokine (C-X-C motif) ligand 12
Alias:
- AI174028
- expressed sequence AI174028
- PBSF
- PBSF/SDF-1
- Scyb12
- SDF-1
- Sdf1
- Sdf1a
- Sdf1b
- stromal cell derived factor 1
- stromal cell derived factor 1A
- stromal cell derived factor 1B
- TLSF-a
- TLSF-b
- TPAR1
NCBI_Gene: 20315
- UniprotKB: P40224
- UniprotKB: H7BX38

Mouse Cxcl12 Predicted Functions

Enables chemokine activity. Involved in dopaminergic neuron differentiation. Acts upstream of or within several processes, including gamete generation; nervous system development; and positive regulation of intracellular signal transduction. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in breast carcinoma; diabetic angiopathy; human immunodeficiency virus infectious disease; and type 1 diabetes mellitus. Orthologous to human CXCL12 (C-X-C motif chemokine ligand 12).

Rat Cxcl12 Target Information

Target Symbol: Cxcl12, C-X-C motif chemokine ligand 12
Alias:
- chemokine (C-X-C motif) ligand 12
- chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
- SDF-1 gamma
- Sdf1
- Stromal cell-derived factor 1
- stromal cell-derived factor-1 gamma
NCBI_Gene: 24772
- UniprotKB: Q80YV8
- UniprotKB: Q9QZD1

Rat Cxcl12 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity; chemokine activity; and integrin binding activity. Involved in several processes, including detection of stimulus involved in sensory perception of pain; nervous system development; and regulation of ion transport. Located in several cellular components, including extracellular space; nucleus; and plasma membrane. Used to study myocardial infarction. Biomarker of anti-basement membrane glomerulonephritis; brain ischemia; myocardial infarction; status epilepticus; and transient cerebral ischemia. Human ortholog(s) of this gene implicated in breast carcinoma; diabetic angiopathy; human immunodeficiency virus infectious disease; and type 1 diabetes mellitus. Orthologous to human CXCL12 (C-X-C motif chemokine ligand 12).

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