Human CXCL13/BLC/BCA-1 ELISA Kit

$350.00$450.00

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TARGETSCXCL13
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.86 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 114%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.86 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 114%
Average Recovery0.99
Intra Precision1.3% – 6.5%
Inter-Precision2.7% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL13/BLC/BCA-1 monoclonal antibody
Human CXCL13/BLC/BCA-1 freeze-dried standard
CXCL13/BLC/BCA-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL13 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL13 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL13 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CXCL13

CXCL13 Target Infomation Overview

  • Target Symbol: CXCL13, C-X-C motif chemokine ligand 13
  • Gene Groups: Chemokine ligands
  • Alias: BLC; BCA-1; BLR1L; ANGIE; ANGIE2
  • Previous Names: SCYB13
  • Alias Names: B-cell chemoattractant; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13

CXCL13, C-X-C motif chemokine ligand 13 Target Infomation by Species

HumanMouseRat

Human CXCL13 Target Information

  • Target Symbol: CXCL13, C-X-C motif chemokine ligand 13
  • Alias:
    • ANGIE
    • ANGIE2
    • b cell-attracting chemokine 1
    • b lymphocyte chemoattractant
    • B-cell chemoattractant
    • B-cell-attracting chemokine 1
    • B-cell-homing chemokine (ligand for Burkitt’s lymphoma receptor-1)
    • B-lymphocyte chemoattractant
    • BCA-1
    • BCA1
    • BLC
    • BLR1L
    • C-X-C motif chemokine 13
    • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
    • CXC chemokine BLC
    • SCYB13
    • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
    • small-inducible cytokine B13
  • NCBI_Gene: 10563
  • UniProtKB: O43927

Human CXCL13 Predicted Functions

Enables chemokine receptor binding activity; fibroblast growth factor binding activity; and heparin binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; cell chemotaxis; and regulation of chemotaxis. Located in extracellular region.

Mouse Cxcl13 Target Information

Mouse Cxcl13 Predicted Functions

Enables CXCR5 chemokine receptor binding activity and chemokine activity. Involved in several processes, including B cell chemotaxis across high endothelial venule; activation of GTPase activity; and lymph node development. Located in extracellular space. Is expressed in Peyer’s patch; aorta; central nervous system; and retina. Orthologous to human CXCL13 (C-X-C motif chemokine ligand 13).

Rat Cxcl13 Target Information

  • Target Symbol: Cxcl13, C-X-C motif chemokine ligand 13
  • Alias:
    • C-X-C motif chemokine 13
    • chemokine (C-X-C motif) ligand 13
    • LOC498335
    • similar to Small inducible cytokine B13 precursor (CXCL13) (B lymphocyte chemoattractant) (CXC chemokine BLC)
  • NCBI_Gene: 498335

Rat Cxcl13 Predicted Functions

Predicted to enable chemokine receptor binding activity; fibroblast growth factor binding activity; and heparin binding activity. Predicted to be involved in several processes, including cell chemotaxis; regulation of chemotaxis; and response to bacterium. Predicted to act upstream of or within lymph node development. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Orthologous to human CXCL13 (C-X-C motif chemokine ligand 13).

CXCL13 Target News

    17.521
    DEX-Induced SREBF1 Promotes BMSCs Differentiation into Adipocytes to Attract and Protect Residual T-Cell Acute Lymphoblastic Leukemia Cells After Chemotherapy
    Advanced Science
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Systemic Lupus Erythematosus

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