Human E-Selectin ELISA Kit

$245.00 – $350.00

Related Target	SELE
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.94 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	84% – 120%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	SELE
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.94 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	84% – 120%
Average Recovery	102%
Intra Precision	4.9% – 5.4%
Inter Precision	3.7% – 7.7%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sE-Selectin monoclonal antibody Human sE-Selectin freeze-dried standard sE-Selectin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sE-Selectin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sE-Selectin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sE-Selectin in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

SELE

SELE Target Infomation Overview

Target Symbol: SELE, selectin E
Gene Groups: CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
Alias: ESEL; CD62E
Previous Names: ELAM1; ELAM
Alias Names: endothelial adhesion molecule 1

SELE, selectin E Target Infomation by Species

Human
Mouse
Rat

Human SELE Target Information

Target Symbol: SELE, selectin E
Alias:
- CD62 antigen-like family member E
- CD62E
- E-selectin
- ELAM
- ELAM-1
- ELAM1
- endothelial adhesion molecule 1
- endothelial leukocyte adhesion molecule 1
- ESEL
- LECAM2
- leukocyte endothelial cell adhesion molecule 2
- leukocyte-endothelial cell adhesion molecule 2
NCBI_Gene: 6401
UniProtKB: P16581

Human SELE Predicted Functions

Enables several functions, including oligosaccharide binding activity; phospholipase binding activity; and sialic acid binding activity. Involved in several processes, including activation of phospholipase C activity; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; and leukocyte tethering or rolling. Located in several cellular components, including caveola; clathrin-coated pit; and perinuclear region of cytoplasm. Is integral component of plasma membrane. Implicated in IgA glomerulonephritis; brain ischemia; cerebrovascular disease; and coronary artery disease. Biomarker of COVID-19; rheumatic myocarditis; and type 1 diabetes mellitus.

Mouse Sele Target Information

Target Symbol: Sele, selectin, endothelial cell
Alias:
- CD62E
- E-selectin
- Elam
- endothelial adhesion molecule
NCBI_Gene: 20339
- UniprotKB: Q00690
- UniprotKB: Q3U5F6

Mouse Sele Predicted Functions

Predicted to enable several functions, including oligosaccharide binding activity; phospholipase binding activity; and sialic acid binding activity. Involved in positive regulation of leukocyte tethering or rolling. Acts upstream of or within positive regulation of leukocyte migration. Predicted to be located in several cellular components, including caveola; clathrin-coated pit; and perinuclear region of cytoplasm. Predicted to be integral component of plasma membrane. Predicted to be active in external side of plasma membrane and extracellular space. Is expressed in several structures, including adrenal gland; genitourinary system; incisor; integumental system; and limb segment. Human ortholog(s) of this gene implicated in IgA glomerulonephritis; brain ischemia; cerebrovascular disease; and coronary artery disease. Orthologous to human SELE (selectin E).

Rat Sele Target Information

Target Symbol: Sele, selectin E
Alias:
- CD62 antigen-like family member E
- E-selectin
- ELAM-1
- endothelial leukocyte adhesion molecule 1
- LECAM2
- leukocyte-endothelial cell adhesion molecule 2
- selectin, endothelial cell
NCBI_Gene: 25544
- UniprotKB: A0A0H2UHU5
- UniprotKB: P98105

Rat Sele Predicted Functions

Predicted to enable several functions, including oligosaccharide binding activity; phospholipase binding activity; and sialic acid binding activity. Involved in inflammatory response; leukocyte migration; and response to cytokine. Predicted to be located in several cellular components, including caveola; clathrin-coated pit; and perinuclear region of cytoplasm. Predicted to be integral component of plasma membrane. Predicted to be active in external side of plasma membrane and extracellular space. Human ortholog(s) of this gene implicated in IgA glomerulonephritis; brain ischemia; cerebrovascular disease; and coronary artery disease. Orthologous to human SELE (selectin E).

Citations for Human E-Selectin ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.