Human Endostatin ELISA Kit

$245.00 – $350.00

Related Target	COL18A1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	6.67 pg/mL
Array Range	62.50 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	81% – 110%
Average Recovery	92%

ELISA Kit Detail Information

Related Target	COL18A1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	6.67 pg/mL
Array Range	62.50 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	81% – 110%
Average Recovery	92%
Intra Precision	1.5% – 2.0%
Inter Precision	1.9% - 2.5%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Endostatin monoclonal antibody Mouse Endostatin freeze-dried standard Endostatin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(22×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Endostatin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Endostatin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Endostatin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

COL18A1

COL18A1 Target Infomation Overview

Target Symbol: COL18A1, collagen type XVIII alpha 1 chain
Gene Groups: Collagens; MicroRNA protein coding host genes
Alias: KS; KNO1
Previous Names: KNO
Alias Names: endostatin; Knobloch syndrome, type 1; collagen, type XVIII, alpha 1

COL18A1, collagen type XVIII alpha 1 chain Target Infomation by Species

Human
Mouse
Rat

Human COL18A1 Target Information

Target Symbol: COL18A1, collagen type XVIII alpha 1 chain
Alias:
- antiangiogenic agent
- collagen alpha-1(XVIII) chain
- collagen alpha-1(XVIII) chain isoform 1 preproprotein
- collagen type XVIII alpha 1
- collagen, type XVIII, alpha 1
- endostatin
- FLJ27325
- FLJ34914
- GLCC
- KNO
- KNO1
- Knobloch syndrome
- KS
- MGC74745
- multi-functional protein MFP
NCBI_Gene: 80781
UniProtKB: P39060

Human COL18A1 Predicted Functions

Enables identical protein binding activity. Predicted to be involved in angiogenesis; endothelial cell morphogenesis; and extracellular matrix organization. Predicted to act upstream of or within positive regulation of cell migration; positive regulation of cell population proliferation; and positive regulation of endothelial cell apoptotic process. Located in extracellular exosome. Colocalizes with collagen-containing extracellular matrix. Implicated in primary angle-closure glaucoma.

Mouse Col18a1 Target Information

Target Symbol: Col18a1, collagen, type XVIII, alpha 1
Alias:
- endostatin
NCBI_Gene: 12822

Mouse Col18a1 Predicted Functions

Predicted to enable identical protein binding activity. Predicted to be an extracellular matrix structural constituent. Acts upstream of or within several processes, including angiogenesis; endothelial cell morphogenesis; and positive regulation of endothelial cell apoptotic process. Located in basement membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Used to study pigment dispersion syndrome. Human ortholog(s) of this gene implicated in primary angle-closure glaucoma. Orthologous to human COL18A1 (collagen type XVIII alpha 1 chain).

Rat Col18a1 Target Information

Target Symbol: Col18a1, collagen type XVIII alpha 1 chain
Alias:
- collagen alpha-1(XVIII) chain
- collagen type XVIII alpha 1
- collagen, type XVIII, alpha 1
- procollagen, type XVIII, alpha 1
NCBI_Gene: 85251
- UniprotKB: F1LR02
- UniprotKB: Q9WUW5

Rat Col18a1 Predicted Functions

Predicted to enable identical protein binding activity. Predicted to be an extracellular matrix structural constituent. Involved in response to hydrostatic pressure and response to xenobiotic stimulus. Located in extracellular space. Used to study hepatocellular carcinoma. Biomarker of gastric ulcer and ulcerative colitis. Human ortholog(s) of this gene implicated in primary angle-closure glaucoma. Orthologous to human COL18A1 (collagen type XVIII alpha 1 chain).

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