Human Fas Ligand/TNFSF6 ELISA Kit

$350.00$450.00

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TARGETSFASLG
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity3.42 pg/mL
Range15.63 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 107%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity3.42 pg/mL
Range15.63 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 107%
Average Recovery0.97
Intra Precision3.0% – 4.2%
Inter-Precision3.9% – 6.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Human Fas Ligand monoclonal antibody
Human Fas Ligand freeze-dried standard
Fas Ligand detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Fas Ligand antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Fas Ligand present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Fas Ligand in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

FASLG

FASLG Target Infomation Overview

  • Target Symbol: FASLG, Fas ligand
  • Gene Groups: CD molecules; Tumor necrosis factor superfamily; Death inducing signaling complex
  • Alias: FasL; CD178
  • Previous Names: APT1LG1; TNFSF6
  • Alias Names: tumor necrosis factor (ligand) superfamily, member 6; Fas ligand (TNF superfamily, member 6)

FASLG, Fas ligand Target Infomation by Species

HumanMouseRat

Human FASLG Target Information

  • Target Symbol: FASLG, Fas ligand
  • Alias:
    • ALPS1B
    • apoptosis (APO-1) antigen ligand 1
    • apoptosis antigen ligand
    • APT1LG1
    • APTL
    • CD178
    • CD95 ligand
    • CD95-L
    • CD95L
    • fas antigen ligand
    • Fas ligand (TNF superfamily, member 6)
    • FASL
    • mutant tumor necrosis factor family member 6
    • TNFSF6
    • TNLG1A
    • tumor necrosis factor (ligand) superfamily, member 6
    • tumor necrosis factor ligand 1A
    • tumor necrosis factor ligand superfamily member 6
  • NCBI_Gene: 356
  • UniProtKB: P48023

Human FASLG Predicted Functions

Predicted to enable signaling receptor binding activity. Involved in several processes, including necroptotic signaling pathway; positive regulation of endothelial cell apoptotic process; and positive regulation of phosphatidylserine exposure on apoptotic cell surface. Acts upstream of or within extrinsic apoptotic signaling pathway via death domain receptors; positive regulation of apoptotic process; and release of sequestered calcium ion into cytosol by endoplasmic reticulum. Located in extracellular exosome; nucleus; and plasma membrane. Implicated in several diseases, including cholangiocarcinoma; chronic myeloid leukemia; pancreatic cancer (multiple); pre-eclampsia (multiple); and type 2 diabetes mellitus. Biomarker of several diseases, including bone marrow cancer (multiple); carcinoma (multiple); cervix uteri carcinoma in situ; clonorchiasis; and neurodegenerative disease (multiple).

Mouse Fasl Target Information

Mouse Fasl Predicted Functions

Predicted to enable death receptor binding activity and tumor necrosis factor receptor binding activity. Acts upstream of or within extrinsic apoptotic signaling pathway via death domain receptors and retinal cell programmed cell death. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; reproductive system; and trophectoderm. Used to study autoimmune lymphoproliferative syndrome and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including cholangiocarcinoma; chronic myeloid leukemia; diabetes mellitus (multiple); pancreatic cancer (multiple); and pre-eclampsia (multiple). Orthologous to human FASLG (Fas ligand).

Rat Faslg Target Information

  • Target Symbol: Faslg, Fas ligand
  • Alias:
    • apoptosis (APO-1) antigen ligand 1 (Fas antigen ligand)
    • Apt1Lg1
    • CD95 ligand
    • CD95-L
    • CD95L protein
    • Fas antigen ligand
    • Fas ligand (TNF superfamily, member 6)
    • Fasl
    • Tnfsf6
    • Tnlg1a
    • Tumor necrosis factor (ligand) superfamily member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand)
    • tumor necrosis factor (ligand) superfamily, member 6
    • Tumor necrosis factor (ligand) superfamily, member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand)
    • tumor necrosis factor ligand 1a
    • tumor necrosis factor ligand superfamily member 6
  • NCBI_Gene: 25385

Rat Faslg Predicted Functions

Enables death receptor binding activity and tumor necrosis factor receptor binding activity. Involved in several processes, including cellular ion homeostasis; cellular response to interferon-gamma; and extrinsic apoptotic signaling pathway via death domain receptors. Located in caveola; cytoplasmic vesicle; and perinuclear region of cytoplasm. Used to study brain glioma; substance-related disorder; and type 1 diabetes mellitus. Biomarker of several diseases, including artery disease (multiple); chronic obstructive pulmonary disease; gastric ulcer; liver disease (multiple); and ulcerative colitis. Human ortholog(s) of this gene implicated in several diseases, including cholangiocarcinoma; chronic myeloid leukemia; diabetes mellitus (multiple); pancreatic cancer (multiple); and pre-eclampsia (multiple). Orthologous to human FASLG (Fas ligand).

FASLG Target News

    Tumor Marker

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