Human Heregulinβ-1 ELISA Kit

$245.00 – $350.00

Related Target	NRG1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	1.54 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 103%
Average Recovery	86%

ELISA Kit Detail Information

Related Target	NRG1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	1.54 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	83% – 103%
Average Recovery	86%
Intra Precision	3.6% – 4.9%
Inter Precision	4.1% - 6.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Heregulinβ-1 monoclonal antibody Human Heregulinβ-1 freeze-dried standard Human Heregulinβ-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Heregulinβ-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Heregulinβ-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Heregulinβ-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

NRG1

NRG1 Target Infomation Overview

Target Symbol: NRG1, neuregulin 1
Gene Groups: I-set domain containing; Neuregulins
Alias: HRG; NDF; GGF
Previous Names: HGL; NRG1-IT2
Alias Names: NRG1 intronic transcript 2 (non-protein coding)

NRG1, neuregulin 1 Target Infomation by Species

Human
Mouse
Rat

Human NRG1 Target Information

Target Symbol: NRG1, neuregulin 1
Alias:
- acetylcholine receptor-inducing activity
- ARIA
- GGF
- GGF2
- glial growth factor
- glial growth factor 2
- heregulin, alpha (45kD, ERBB2 p185-activator)
- HGL
- HRG
- HRG1
- HRGA
- MST131
- MSTP131
- NDF
- neu differentiation factor
- neuregulin 1 type IV beta 1a
- neuregulin 1 type IV beta 3
- NRG1 intronic transcript 2 (non-protein coding)
- NRG1-IT2
- pro-neuregulin-1, membrane-bound isoform
- pro-NRG1
- sensory and motor neuron derived factor
- SMDF
NCBI_Gene: 3084
UniProtKB: Q02297

Human NRG1 Predicted Functions

Enables protein tyrosine kinase activator activity; signaling receptor binding activity; and transcription coregulator activity. Involved in several processes, including ERBB signaling pathway; heart development; and positive regulation of protein phosphorylation. Acts upstream of or within cellular protein complex disassembly and negative regulation of transcription, DNA-templated. Located in apical plasma membrane; extracellular space; and nucleoplasm. Is active in glutamatergic synapse. Is integral component of postsynaptic density membrane and integral component of presynaptic active zone membrane. Implicated in several diseases, including bipolar disorder; cerebral malaria; dementia; middle cerebral artery infarction; and psychotic disorder (multiple). Biomarker of Alzheimer’s disease; hepatocellular carcinoma; major depressive disorder; and ovarian cancer.

Mouse Nrg1 Target Information

Target Symbol: Nrg1, neuregulin 1
Alias:
- 6030402G23Rik
- ARIA
- D230005F13Rik
- GGF
- GGFII
- heregulin
- Hgl
- HGL
- HRG
- HRGalpha
- NDF
- RIKEN cDNA 6030402G23 gene
- RIKEN cDNA D230005F13 gene
- SMDF
NCBI_Gene: 211323

Mouse Nrg1 Predicted Functions

Enables ErbB-2 class receptor binding activity and chemorepellent activity. Involved in several processes, including positive regulation of cardiac muscle cell differentiation; postsynapse to nucleus signaling pathway; and synapse assembly. Acts upstream of or within several processes, including neurogenesis; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in several cellular components, including axolemma; external side of plasma membrane; and neuromuscular junction. Is integral component of plasma membrane. Is active in GABA-ergic synapse and glutamatergic synapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and sensory organ. Used to study schizophrenia. Human ortholog(s) of this gene implicated in several diseases, including bipolar disorder; cerebral malaria; dementia; middle cerebral artery infarction; and psychotic disorder (multiple). Orthologous to human NRG1 (neuregulin 1).

Rat Nrg1 Target Information

Target Symbol: Nrg1, neuregulin 1
Alias:
- glial growth factor
- neuregulin 1 type III beta 3
- pro-neuregulin-1, membrane-bound isoform
- pro-NRG1
NCBI_Gene: 112400

Rat Nrg1 Predicted Functions

Enables receptor tyrosine kinase binding activity. Involved in several processes, including negative regulation of corticosterone secretion; positive regulation of Schwann cell proliferation; and response to progesterone. Located in extracellular space and synapse. Used to study sciatic neuropathy. Biomarker of diabetic neuropathy; median neuropathy; schizophrenia; and visual epilepsy. Human ortholog(s) of this gene implicated in several diseases, including bipolar disorder; cerebral malaria; dementia; middle cerebral artery infarction; and psychotic disorder (multiple). Orthologous to human NRG1 (neuregulin 1).

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