Human HGF ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 1.99 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 108% |
| Average Recovery | 95% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 1.99 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 108% |
| Average Recovery | 95% |
| Intra Precision | 4.3% – 6.5% |
| Inter Precision | 3.1% – 8.3% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-HGF monoclonal antibody Human HGF freeze-dried standard HGF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human HGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, HGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of HGF in the samples. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
HGF
HGF Target Infomation Overview
- Target Symbol: HGF, hepatocyte growth factor
- Alias: SF; F-TCF; HGFB; HPTA
- Previous Names: DFNB39
- Alias Names: hepatopoietin A; fibroblast-derived tumor cytotoxic factor; scatter factor; lung fibroblast-derived mitogen; deafness, autosomal recessive 39; hepatocyte growth factor (hepapoietin A; scatter factor)
HGF, hepatocyte growth factor Target Infomation by Species
- Human
- Mouse
- Rat
Human HGF Target Information
- Target Symbol: HGF, hepatocyte growth factor
- Alias:
- deafness, autosomal recessive 39
- DFNB39
- F-TCF
- fibroblast-derived tumor cytotoxic factor
- hepatocyte growth factor (hepapoietin A, scatter factor)
- hepatopoeitin-A
- hepatopoietin-A
- HGFB
- HPTA
- lung fibroblast-derived mitogen
- SF
- NCBI_Gene: 3082
- UniProtKB: P14210
Human HGF Predicted Functions
Enables chemoattractant activity and identical protein binding activity. Involved in several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of programmed cell death; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling. Located in membrane. Implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Biomarker of several diseases, including artery disease (multiple); biliary tract disease (multiple); myositis (multiple); retinal disease (multiple); and rheumatoid arthritis (multiple).
Mouse Hgf Target Information
- Target Symbol: Hgf, hepatocyte growth factor
- Alias:
- C230052L06Rik
- HGF/SF
- NK1
- NK2
- RIKEN cDNA C230052L06 gene
- scatter factor
- SF/HGF
- NCBI_Gene: 15234
Mouse Hgf Predicted Functions
Predicted to enable chemoattractant activity; growth factor activity; and identical protein binding activity. Acts upstream of or within several processes, including regulation of MAPK cascade; regulation of cytokine production; and regulation of protein phosphorylation. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; egg cylinder; embryo mesenchyme; and genitourinary system. Used to study autosomal recessive nonsyndromic deafness 39 and breast cancer. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Orthologous to human HGF (hepatocyte growth factor).
Rat Hgf Target Information
- Target Symbol: Hgf, hepatocyte growth factor
- Alias:
- hepatocyte growth factor (scatter factor)
- hepatopoeitin-A
- hepatopoietin-A
- HPTA
- scatter factor
- SF
- NCBI_Gene: 24446
- UniProtKB: P17945
Rat Hgf Predicted Functions
Enables growth factor activity. Involved in several processes, including animal organ regeneration; positive regulation of angiogenesis; and positive regulation of myelination. Located in extracellular space. Used to study brain infarction. Biomarker of cholangiocarcinoma. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Orthologous to human HGF (hepatocyte growth factor).
Products with the Same Target - HGF
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