Human IgA ELISA Kit

$350.00$450.00

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SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL;cell culture supernatant: 100 μL
Sensitivity0.35 ng/mL
Range3.13 ng/mL – 200 ng/mL
Assay Time3.5 h
Recovery90% – 108%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL;cell culture supernatant: 100 μL
Sensitivity0.35 ng/mL
Range3.13 ng/mL – 200 ng/mL
Assay Time3.5 h
Recovery90% – 108%
Average Recovery0.97
Intra Precision3.4% – 4.8%
Inter-Precision0.7% – 6.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgA monoclonal antibody
Human IgA freeze-dried standard
IgA detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IgA antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IgA present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgA in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

8.786
A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide.
Frontiers in Immunology
7.561
Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis
Frontiers in Immunology

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