Human IGFBP-2 ELISA Kit

$350.00$450.00

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TARGETSIGFBP2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.19 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery98% – 116%
Average Recovery1.06
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.19 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery98% – 116%
Average Recovery1.06
Intra Precision1.7% – 4.3%
Inter-Precision2.3% – 3.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TRAIL/TNFSF10 monoclonal antibody
Human TRAIL/TNFSF10 freeze-dried standard
TRAIL/TNFSF10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGFBP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IGFBP2

IGFBP2 Target Infomation Overview

  • Target Symbol: IGFBP2, insulin like growth factor binding protein 2
  • Gene Groups: Insulin like growth factor binding proteins
  • Previous Names: IBP2
  • Alias Names: insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor binding protein 2

IGFBP2, insulin like growth factor binding protein 2 Target Infomation by Species

HumanMouseRat

Human IGFBP2 Target Information

  • Target Symbol: IGFBP2, insulin like growth factor binding protein 2
  • Alias:
    • IBP-2
    • IBP2
    • IGF-binding protein 2
    • IGF-BP53
    • IGFBP-2
    • insulin-like growth factor binding protein 2
    • insulin-like growth factor binding protein 2, 36kDa
    • insulin-like growth factor-binding protein 2
  • NCBI_Gene: 3485
  • UniProtKB: P18065

Human IGFBP2 Predicted Functions

Enables insulin-like growth factor I binding activity. Involved in positive regulation of activated T cell proliferation. Located in extracellular space. Implicated in obesity. Biomarker of Alzheimer’s disease; Kuhnt-Junius degeneration; and obesity.

Mouse Igfbp2 Target Information

Mouse Igfbp2 Predicted Functions

Enables insulin-like growth factor binding activity. Predicted to be involved in positive regulation of activated T cell proliferation and regulation of insulin-like growth factor receptor signaling pathway. Predicted to act upstream of or within regulation of growth. Located in extracellular space. Is expressed in several structures, including brain; early embryo; limb; metanephros; and reproductive system. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP2 (insulin like growth factor binding protein 2).

Rat Igfbp2 Target Information

  • Target Symbol: Igfbp2, insulin-like growth factor binding protein 2
  • Alias:
    • BRL-BP
    • IBP-2
    • IGF-binding protein 2
    • IGFBP-2
    • ILGFBPA
    • insulin-like growth factor-binding protein 2
  • NCBI_Gene: 25662
  • UniProtKB: P12843

Rat Igfbp2 Predicted Functions

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in several processes, including response to estradiol; response to estrogen; and response to retinoic acid. Located in apical plasma membrane; cytoplasmic vesicle; and extracellular space. Biomarker of hypertension and hypothyroidism. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP2 (insulin like growth factor binding protein 2).

IGFBP2 Target News

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