Mouse IGFBP-2 ELISA Kit

EK2278

Mouse IGFBP-2 ELISA Kit

$245.00 – $350.00

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity6.13 pg/mL
Array Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery88% – 103%
Average Recovery94%

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity6.13 pg/mL
Array Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery88% – 103%
Average Recovery94%
Intra Precision3.2% – 4.6%
Inter Precision3.7% - 4.8%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-2 monoclonal antibody
Mouse IGFBP-2 freeze-dried standard
Mouse IGFBP-2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IGFBP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGFBP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGFBP2

IGFBP2 Target Infomation Overview

  • Target Symbol: IGFBP2, insulin like growth factor binding protein 2
  • Gene Groups: Insulin like growth factor binding proteins
  • Previous Names: IBP2
  • Alias Names: insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor binding protein 2

IGFBP2, insulin like growth factor binding protein 2 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IGFBP2 Target Information

  • Target Symbol: IGFBP2, insulin like growth factor binding protein 2
  • Alias:
    • IBP-2
    • IBP2
    • IGF-binding protein 2
    • IGF-BP53
    • IGFBP-2
    • insulin-like growth factor binding protein 2
    • insulin-like growth factor binding protein 2, 36kDa
    • insulin-like growth factor-binding protein 2
  • NCBI_Gene: 3485
  • UniProtKB: P18065

Human IGFBP2 Predicted Functions

Enables insulin-like growth factor I binding activity. Involved in positive regulation of activated T cell proliferation. Located in extracellular space. Implicated in obesity. Biomarker of Alzheimer’s disease; Kuhnt-Junius degeneration; and obesity.

Mouse Igfbp2 Target Information

Mouse Igfbp2 Predicted Functions

Enables insulin-like growth factor binding activity. Predicted to be involved in positive regulation of activated T cell proliferation and regulation of insulin-like growth factor receptor signaling pathway. Predicted to act upstream of or within regulation of growth. Located in extracellular space. Is expressed in several structures, including brain; early embryo; limb; metanephros; and reproductive system. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP2 (insulin like growth factor binding protein 2).

Rat Igfbp2 Target Information

  • Target Symbol: Igfbp2, insulin-like growth factor binding protein 2
  • Alias:
    • BRL-BP
    • IBP-2
    • IGF-binding protein 2
    • IGFBP-2
    • ILGFBPA
    • insulin-like growth factor-binding protein 2
  • NCBI_Gene: 25662
  • UniProtKB: P12843

Rat Igfbp2 Predicted Functions

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in several processes, including response to estradiol; response to estrogen; and response to retinoic acid. Located in apical plasma membrane; cytoplasmic vesicle; and extracellular space. Biomarker of hypertension and hypothyroidism. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP2 (insulin like growth factor binding protein 2).

Products with the Same Target - IGFBP2

Human IGFBP-2 ELISA Kit

$245.00 – $350.00

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Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

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