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Human IGFBP-6 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 8.38 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 87% – 103% |
| Average Recovery | 0.96 |
| Intra Precision | 0.8% – 1.2% |
| Inter-Precision | 0.8% – 2.0% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-6 monoclonal antibody Human IGFBP-6 freeze-dried standard IGFBP-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGFBP-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-6 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IGFBP6
IGFBP6 Target Infomation Overview
- Target Symbol: IGFBP6, insulin like growth factor binding protein 6
- Gene Groups: Insulin like growth factor binding proteins
- Alias Names: insulin-like growth factor binding protein 6
IGFBP6, insulin like growth factor binding protein 6 Target Infomation by Species
HumanMouseRat
Human IGFBP6 Target Information
- Target Symbol: IGFBP6, insulin like growth factor binding protein 6
- Alias:
- IBP-6
- IBP6
- IGF binding protein 6
- IGF-binding protein 6
- IGFBP-6
- insulin-like growth factor binding protein 6
- insulin-like growth factor-binding protein 6
- NCBI_Gene: 3489
- UniProtKB: P24592
Human IGFBP6 Predicted Functions
Enables identical protein binding activity and insulin-like growth factor II binding activity. Involved in cell migration and positive regulation of stress-activated MAPK cascade. Part of insulin-like growth factor binary complex. Implicated in obesity. Biomarker of breast cancer; in situ carcinoma; leiomyoma; and neovascular inflammatory vitreoretinopathy.
Mouse Igfbp6 Target Information
- Target Symbol: Igfbp6, insulin-like growth factor binding protein 6
- Alias:
- IGFBP-6
- NCBI_Gene: 16012
Mouse Igfbp6 Predicted Functions
Predicted to enable fibronectin binding activity; identical protein binding activity; and insulin-like growth factor binding activity. Predicted to be involved in cell migration; positive regulation of stress-activated MAPK cascade; and regulation of insulin-like growth factor receptor signaling pathway. Located in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including blastocyst; compacted morula; genitourinary system; liver; and spleen. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP6 (insulin like growth factor binding protein 6).
Rat Igfbp6 Target Information
- Target Symbol: Igfbp6, insulin-like growth factor binding protein 6
- Alias:
- IBP-6
- IGF-binding protein 6
- IGF-BP6
- IGFBP-6
- insulin-like growth factor-binding protein 6
- RBP6A
- RBP6G
- NCBI_Gene: 25641
- UniProtKB: P35572
Rat Igfbp6 Predicted Functions
Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in cell migration; positive regulation of stress-activated MAPK cascade; and regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus and extracellular space. Human ortholog(s) of this gene implicated in obesity. Orthologous to human IGFBP6 (insulin like growth factor binding protein 6).
