Human IL-1β ELISA Kit

$350.00$450.00

TARGETS IL1B
SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.15 pg/mL
Range 3.91 pg/mL – 250 pg/mL
Assay Time 3.5 h
Recovery 94% – 113%
Average Recovery 1.05
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.15 pg/mL
Range 3.91 pg/mL – 250 pg/mL
Assay Time 3.5 h
Recovery 94% – 113%
Average Recovery 1.05
Intra Precision 4.7% – 6.1%
Inter-Precision 4.1% – 6.6%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1β monoclonal antibody
Human IL-1β freeze-dried standard
IL-1β detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-1β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-1β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL1B

IL1B Target Infomation Overview

  • Target Symbol: IL1B, interleukin 1 beta
  • Gene Groups: Interleukins
  • Alias: IL1F2; IL-1B; IL1-BETA

IL1B, interleukin 1 beta Target Infomation by Species

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Human IL1B Target Information

  • Target Symbol: IL1B, interleukin 1 beta
  • Alias:
    • catabolin
    • IL-1
    • IL-1 beta
    • IL1-BETA
    • IL1beta
    • IL1F2
    • interleukin 1, beta
    • interleukin 1beta
    • interleukin-1 beta
    • preinterleukin 1 beta
    • pro-interleukin-1-beta
  • NCBI_Gene: 3553
  • UniProtKB: P01584

Human IL1B Predicted Functions

Enables cytokine activity; integrin binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of nitrogen compound metabolic process; and regulation of signal transduction. Acts upstream of or within cellular response to organic substance and cellular response to xenobiotic stimulus. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bronchial disease (multiple); lung disease (multiple); and nose disease (multiple).

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Mouse Il1b Target Information

Mouse Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

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Rat Il1b Target Information

Rat Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

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IL1B Target News

[catlist tags=”il1b” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

4.8
GDF11 promotes osteogenic/odontogenic differentiation of dental pulp stem cells to accelerate dentin restoration via modulating SIRT3/FOXO3-mediated mitophagy
INTERNATIONAL IMMUNOPHARMACOLOGY
4.4
Pericentriolar material 1 promotes intestinal inflammation in ulcerative colitis by activating NLRP3/gasdermin D-mediated macrophage pyroptosis
FASEB JOURNAL
20.5
Taurolithocholic acid protects against viral haemorrhagic fever via inhibition of ferroptosis
Nature Microbiology
10
Simplified Gambogic Acid Prodrug Nanoparticles to Improve Efficiency and Reduce Toxicity for Clinical Translation Potential
Advanced Healthcare Materials
3.2
METTL3-mediated TIM1 promotes macrophage M1 polarization and inflammation through IGF2BP2-dependent manner
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
2.6
Invasion of human dental pulp fibroblasts by Porphyromonas gingivalis leads to autophagy via the phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling pathway
Journal of Oral Biosciences
7.1
A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation
FREE RADICAL BIOLOGY AND MEDICINE
4.5
Identification of O-arylated huperzinines as novel cholinergic anti-inflammatory pathway agonists against gout arthritis
BIOORGANIC CHEMISTRY
6.8
Discovery of a Covalent Inhibitor of Pro-Caspase-1 Zymogen Blocking NLRP3 Inflammasome Activation and Pyroptosis
JOURNAL OF MEDICINAL CHEMISTRY
4.1
MicroRNA hsa-let-7e-5p in hUC-MSC-EVs alleviates oral mucositis by targeting TAB2
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
3.4
Quercetin Alleviates LPS-Stimulated Myocardial Injury through Regulating ALOX5/PI3K/AKT Pathway in Sepsis
Cardiovascular Toxicology
11.7
Beta2 adrenergic receptor–mediated abnormal myelopoiesis drives neuroinflammation in aged patients with traumatic brain injury
Science Advances
4.8
The protective role of Cordyceps cicadae and its active ingredient myriocin against sodium iodate-induced age-related macular degeneration via an anti-necroptotic TNF-RIPK1/3 pathway
JOURNAL OF ETHNOPHARMACOLOGY
4.2
FOXQ1, deubiquitinated by USP10, alleviates sepsis-induced acute kidney injury by targeting the CREB5/NF-κB signaling axis
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
3
Mesangial cell-derived CircRNAs in chronic glomerulonephritis: RNA sequencing and bioinformatics analysis
RENAL FAILURE
2.3
LncRNA lnc-SPRR2G-2 contributes to keratinocyte hyperproliferation and inflammation in psoriasis by activating the STAT3 pathway and downregulating KHSRP
MOLECULAR AND CELLULAR PROBES
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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