EK113

Human IL-13 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.90 pg/mL
Array Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery82% – 110%
Average Recovery98%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.90 pg/mL
Array Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery82% – 110%
Average Recovery98%
Intra Precision1.7% – 2.0%
Inter Precision2.2% – 4.1%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-13 monoclonal antibody
Human IL-13 freeze-dried standard
IL-13 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-13 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-13 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-13 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL13

IL13 Target Infomation Overview

  • Target Symbol: IL13, interleukin 13
  • Gene Groups: Interleukins
  • Alias: P600; IL-13; ALRH; BHR1; MGC116786; MGC116788; MGC116789
  • Alias Names: allergic rhinitis; Bronchial hyperresponsiveness-1 (bronchial asthma)

IL13, interleukin 13 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL13 Target Information

  • Target Symbol: IL13, interleukin 13
  • Alias:
    • allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness-1 (bronchial asthma)
    • IL-13
    • interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • P600
  • NCBI_Gene: 3596
  • UniProtKB: P35225

Human IL13 Predicted Functions

Predicted to enable cytokine activity and interleukin-13 receptor binding activity. Involved in several processes, including negative regulation of complement-dependent cytotoxicity; negative regulation of endothelial cell apoptotic process; and positive regulation of interleukin-10 production. Located in extracellular region. Implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Biomarker of several diseases, including Plasmodium falciparum malaria; autoimmune disease (multiple); cystic fibrosis; lung disease (multiple); and scleroderma (multiple).

Mouse Il13 Target Information

Mouse Il13 Predicted Functions

Predicted to enable interleukin-13 receptor binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of macrophage activation. Acts upstream of or within cellular response to cytokine stimulus; inflammatory response; and positive regulation of immune effector process. Located in cytoplasm; external side of plasma membrane; and extracellular space. Is expressed in gut; liver; male reproductive gland or organ; and thymus. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).

Rat Il13 Target Information

Rat Il13 Predicted Functions

Enables interleukin-13 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of cation transmembrane transport. Located in extracellular space. Used to study asthma; extrinsic allergic alveolitis; and lipoid nephrosis. Biomarker of several diseases, including alcoholic hepatitis; middle cerebral artery infarction; peptic esophagitis; prostatic hypertrophy; and pulmonary fibrosis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).

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