Human IL-15 ELISA Kit
$299.00 – $419.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 50 μL |
| Sensitivity | 3.78 pg/mL |
| Array Range | 3.13 pg/mL – 200 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 87% – 111% |
| Average Recovery | 97% |
| Intra Precision | 7.1% – 9.0% |
| Inter Precision | 7.8% – 9.5% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-15 monoclonal antibody Human IL-15 freeze-dried standard IL-15 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-15 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-15 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-15 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL15
IL15 Target Infomation Overview
- Target Symbol: IL15, interleukin 15
- Gene Groups: Interleukins
- Alias: IL-15; MGC9721
IL15, interleukin 15 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL15 Target Information
- Target Symbol: IL15, interleukin 15
- Alias:
- IL-15
- interleukin-15
- MGC9721
- NCBI_Gene: 3600
- UniProtKB: P40933
Human IL15 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; macrophage differentiation; and positive regulation of macromolecule metabolic process. Located in cytosol and nuclear speck. Implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Biomarker of several diseases, including artery disease (multiple); brain infarction; lung disease (multiple); obesity; and viral infectious disease (multiple).
Mouse Il15 Target Information
- Target Symbol: Il15, interleukin 15
- Alias:
- AI503618
- expressed sequence AI503618
- NCBI_Gene: 16168
Mouse Il15 Predicted Functions
Enables cytokine activity. Involved in negative regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including T cell activation; lymph node development; and regulation of lymphocyte activation. Located in cytoplasm. Is expressed in several structures, including adrenal gland; genitourinary system; gut; immune system; and nervous system. Human ortholog(s) of this gene implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Orthologous to human IL15 (interleukin 15).
Rat Il15 Target Information
- Target Symbol: Il15, interleukin 15
- Alias:
- IL-15
- interleukin-15
- NCBI_Gene: 25670
- UniProtKB: P97604
Rat Il15 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including cellular response to vitamin D; positive regulation of tyrosine phosphorylation of STAT protein; and skeletal muscle atrophy. Located in cell surface and extracellular space. Used to study pancreatitis. Biomarker of listeriosis and periodontal disease. Human ortholog(s) of this gene implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Orthologous to human IL15 (interleukin 15).
Products with the Same Target - IL15
Citations for Human IL-15 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK1188
- ELISA Kit
Human B4GALT1/GGTB2 ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1215
- ELISA Kit
Human Angiopoietin-2/ANG-2 ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK195
- ELISA Kit
Human Adiponectin ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1109
- ELISA Kit
Human sTNF RI/TNFRSF1A ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1316
- ELISA Kit
Human Arginase 1/ARG1 ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1292
- ELISA Kit
Human ANGPTL4 ELISA Kit
- $299.00 – $419.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.