Human IL-27 ELISA Kit

$350.00$450.00

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TARGETSIL27
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity191.53 pg/mL
Range0.47 ng/mL – 30 ng/mL
Assay Time3.5 h
Recovery80% – 120%
Average Recovery0.96
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity191.53 pg/mL
Range0.47 ng/mL – 30 ng/mL
Assay Time3.5 h
Recovery80% – 120%
Average Recovery0.96
Intra Precision3.8% – 4.4%
Inter-Precision2.9% – 5.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-27 monoclonal antibody
Human IL-27 freeze-dried standard
IL-27 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-27 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-27 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-27 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL27

IL27 Target Infomation Overview

  • Target Symbol: IL27, interleukin 27
  • Gene Groups: Interleukins
  • Alias: IL-27; p28; IL27p28; IL-27A; IL27A; MGC71873
  • Previous Names: IL30
  • Alias Names: interleukin 30

IL27, interleukin 27 Target Infomation by Species

HumanMouseRat

Human IL27 Target Information

  • Target Symbol: IL27, interleukin 27
  • Alias:
    • IL-27
    • IL-27 p28 subunit
    • IL-27 subunit alpha
    • IL-27-A
    • IL-27A
    • IL27-A
    • IL27A
    • IL27p28
    • IL30
    • interleukin 30
    • interleukin-27 subunit alpha
    • interleukin-30
    • MGC71873
    • p28
  • NCBI_Gene: 246778
  • UniProtKB: Q8NEV9

Human IL27 Predicted Functions

Predicted to enable interleukin-27 receptor binding activity. Involved in positive regulation of interferon-gamma production and regulation of T-helper 1 cell differentiation. Predicted to be located in cytosol; endoplasmic reticulum lumen; and extracellular region. Predicted to be active in extracellular space. Implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Biomarker of several diseases, including Henoch-Schoenlein purpura; hepatitis B; infectious mononucleosis; peptic ulcer disease (multiple); and tuberculosis (multiple).

Mouse Il27 Target Information

Mouse Il27 Predicted Functions

Enables interleukin-27 receptor binding activity. Acts upstream of or within positive regulation of defense response to virus by host and regulation of T cell proliferation. Located in extracellular space. Is expressed in decidua; embryo; and extraembryonic component. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Orthologous to human IL27 (interleukin 27).

Rat Il27 Target Information

Rat Il27 Predicted Functions

Predicted to enable interleukin-27 receptor binding activity. Involved in response to bacterium. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Orthologous to human IL27 (interleukin 27).

IL27 Target News

    Multiple Sclerosis
    Systemic Lupus Erythematosus

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