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Human IL-27 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 50 μL；cell culture supernatant: 100 μL |
| Sensitivity | 191.53 pg/mL |
| Range | 0.47 ng/mL – 30 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 120% |
| Average Recovery | 0.96 |
| Intra Precision | 3.8% – 4.4% |
| Inter-Precision | 2.9% – 5.0% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-27 monoclonal antibody Human IL-27 freeze-dried standard IL-27 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-27 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-27 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-27 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL27
IL27 Target Infomation Overview
- Target Symbol: IL27, interleukin 27
- Gene Groups: Interleukins
- Alias: IL-27; p28; IL27p28; IL-27A; IL27A; MGC71873
- Previous Names: IL30
- Alias Names: interleukin 30
IL27, interleukin 27 Target Infomation by Species
HumanMouseRat
Human IL27 Target Information
- Target Symbol: IL27, interleukin 27
- Alias:
- IL-27
- IL-27 p28 subunit
- IL-27 subunit alpha
- IL-27-A
- IL-27A
- IL27-A
- IL27A
- IL27p28
- IL30
- interleukin 30
- interleukin-27 subunit alpha
- interleukin-30
- MGC71873
- p28
- NCBI_Gene: 246778
- UniProtKB: Q8NEV9
Human IL27 Predicted Functions
Predicted to enable interleukin-27 receptor binding activity. Involved in positive regulation of interferon-gamma production and regulation of T-helper 1 cell differentiation. Predicted to be located in cytosol; endoplasmic reticulum lumen; and extracellular region. Predicted to be active in extracellular space. Implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Biomarker of several diseases, including Henoch-Schoenlein purpura; hepatitis B; infectious mononucleosis; peptic ulcer disease (multiple); and tuberculosis (multiple).
Mouse Il27 Target Information
- Target Symbol: Il27, interleukin 27
- Alias:
- IL-27
- IL-27p28
- Il30
- interleukin 30
- p28
- NCBI_Gene: 246779
Mouse Il27 Predicted Functions
Enables interleukin-27 receptor binding activity. Acts upstream of or within positive regulation of defense response to virus by host and regulation of T cell proliferation. Located in extracellular space. Is expressed in decidua; embryo; and extraembryonic component. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Orthologous to human IL27 (interleukin 27).
Rat Il27 Target Information
- Target Symbol: Il27, interleukin 27
- Alias:
- interleukin-27 subunit alpha
- LOC365368
- RGD1561420
- similar to IL-27 p28 subunit
- NCBI_Gene: 365368
- UniProtKB: D3ZPW3
Rat Il27 Predicted Functions
Predicted to enable interleukin-27 receptor binding activity. Involved in response to bacterium. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Orthologous to human IL27 (interleukin 27).
