Human IL-27 ELISA Kit
$245.00 – $350.00
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | Serum, plasma: 50 μL, cell culture supernatant: 100 μL |
Sensitivity | 191.53 pg/mL |
Array Range | 0.47 ng/mL – 30 ng/mL |
Assay Time | 3.5 h |
Recovery | 80% – 120% |
Average Recovery | 96% |
ELISA Kit Detail Information
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | Serum, plasma: 50 μL, cell culture supernatant: 100 μL |
Sensitivity | 191.53 pg/mL |
Array Range | 0.47 ng/mL – 30 ng/mL |
Assay Time | 3.5 h |
Recovery | 80% – 120% |
Average Recovery | 96% |
Intra Precision | 3.8% – 4.4% |
Inter Precision | 2.9% – 5.0% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-27 monoclonal antibody Human IL-27 freeze-dried standard IL-27 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-27 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-27 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-27 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL27
IL27 Target Infomation Overview
- Target Symbol: IL27, interleukin 27
- Gene Groups: Interleukins
- Alias: IL-27; p28; IL27p28; IL-27A; IL27A; MGC71873
- Previous Names: IL30
- Alias Names: interleukin 30
IL27, interleukin 27 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL27 Target Information
- Target Symbol: IL27, interleukin 27
- Alias:
- IL-27
- IL-27 p28 subunit
- IL-27 subunit alpha
- IL-27-A
- IL-27A
- IL27-A
- IL27A
- IL27p28
- IL30
- interleukin 30
- interleukin-27 subunit alpha
- interleukin-30
- MGC71873
- p28
- NCBI_Gene: 246778
- UniProtKB: Q8NEV9
Human IL27 Predicted Functions
Predicted to enable interleukin-27 receptor binding activity. Involved in positive regulation of interferon-gamma production and regulation of T-helper 1 cell differentiation. Predicted to be located in cytosol; endoplasmic reticulum lumen; and extracellular region. Predicted to be active in extracellular space. Implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Biomarker of several diseases, including Henoch-Schoenlein purpura; hepatitis B; infectious mononucleosis; peptic ulcer disease (multiple); and tuberculosis (multiple).
Mouse Il27 Target Information
- Target Symbol: Il27, interleukin 27
- Alias:
- IL-27
- IL-27p28
- Il30
- interleukin 30
- p28
- NCBI_Gene: 246779
Mouse Il27 Predicted Functions
Enables interleukin-27 receptor binding activity. Acts upstream of or within positive regulation of defense response to virus by host and regulation of T cell proliferation. Located in extracellular space. Is expressed in decidua; embryo; and extraembryonic component. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Orthologous to human IL27 (interleukin 27).
Rat Il27 Target Information
- Target Symbol: Il27, interleukin 27
- Alias:
- interleukin-27 subunit alpha
- LOC365368
- RGD1561420
- similar to IL-27 p28 subunit
- NCBI_Gene: 365368
- UniProtKB: D3ZPW3
Rat Il27 Predicted Functions
Predicted to enable interleukin-27 receptor binding activity. Involved in response to bacterium. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; and pulmonary tuberculosis. Orthologous to human IL27 (interleukin 27).
Citations for Human IL-27 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Reviews
There are no reviews yet.