Name: Human IL-6 Rα ELISA Kit
SKU: EK1153
Availability: InStock

EK1153

Human IL-6 Rα ELISA Kit

$245.00 – $350.00

Related Target	IL6R
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.16 pg/mL
Array Range	15.63 ng/mL – 1000 ng/mL
Assay Time	3.5 h
Recovery	90% – 117%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	IL6R
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.16 pg/mL
Array Range	15.63 ng/mL – 1000 ng/mL
Assay Time	3.5 h
Recovery	90% – 117%
Average Recovery	102%
Intra Precision	3.2% – 4.4%
Inter Precision	2.8% - 4.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 Rα monoclonal antibody Human IL-6 Rα freeze-dried standard Human IL-6 Rα detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-6 Rα antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6 Rα present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 Rα in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL6R

IL6R Target Infomation Overview

Target Symbol: IL6R, interleukin 6 receptor
Gene Groups: CD molecules; Immunoglobulin like domain containing; Interleukin receptors
Alias: CD126; IL-6R; IL-1Ra; IL6RA; gp80
Alias Names: membrane glycoprotein 80; interleukin 6 receptor subunit alpha

IL6R, interleukin 6 receptor Target Infomation by Species

Human
Mouse
Rat

Human IL6R Target Information

Target Symbol: IL6R, interleukin 6 receptor
Alias:
- CD126
- CD126 antigen
- gp80
- HIES5
- IL-1Ra
- IL-6 receptor subunit alpha
- IL-6R
- IL-6R 1
- IL-6R subunit alpha
- IL-6R-1
- IL-6R-alpha
- IL-6RA
- IL6Q
- IL6QTL
- IL6RA
- IL6RQ
- interleukin 6 receptor alpha subunit
- interleukin-6 receptor subunit alpha
- membrane glycoprotein 80
- MGC104991
NCBI_Gene: 3570
UniProtKB: P08887

Human IL6R Predicted Functions

Enables cytokine binding activity; enzyme binding activity; and protein homodimerization activity. Contributes to ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity; and interleukin-6 receptor binding activity. Involved in several processes, including cytokine-mediated signaling pathway; positive regulation of cell population proliferation; and positive regulation of macromolecule metabolic process. Located in apical plasma membrane and extracellular space. Part of ciliary neurotrophic factor receptor complex and interleukin-6 receptor complex. Implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Biomarker of several diseases, including Graves’ disease; obesity; proliferative diabetic retinopathy; pulmonary tuberculosis; and sleep apnea.

Mouse Il6ra Target Information

Target Symbol: Il6ra, interleukin 6 receptor, alpha
Alias:
- CD126
- IL-6 receptor alpha chain
- IL-6R
- Il6r
- interleukin 6 receptor
NCBI_Gene: 16194

Mouse Il6ra Predicted Functions

Enables interleukin-6 binding activity and interleukin-6 receptor activity. Involved in T-helper 17 cell lineage commitment and interleukin-6-mediated signaling pathway. Located in cell surface. Part of interleukin-6 receptor complex. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system; and nervous system. Human ortholog(s) of this gene implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Orthologous to human IL6R (interleukin 6 receptor).

Rat Il6r Target Information

Target Symbol: Il6r, interleukin 6 receptor
Alias:
- IL-6 receptor subunit alpha
- IL-6R 1
- IL-6R subunit alpha
- IL-6R-alpha
- IL-6RA
- IL6R1
- Il6ra
- interleukin 6 receptor, alpha
- interleukin-6 receptor subunit alpha
NCBI_Gene: 24499
- UniprotKB: G3V8T6
- UniprotKB: P22273

Rat Il6r Predicted Functions

Predicted to enable several functions, including cytokine binding activity; cytokine receptor activity; and protein homodimerization activity. Predicted to contribute to ciliary neurotrophic factor receptor activity and interleukin-6 receptor binding activity. Involved in several processes, including response to cAMP; response to gamma radiation; and response to lipopolysaccharide. Located in extracellular space and neuronal cell body membrane. Biomarker of several diseases, including congestive heart failure; mental depression; orchitis; sciatic neuropathy; and visual epilepsy. Human ortholog(s) of this gene implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Orthologous to human IL6R (interleukin 6 receptor).

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