EK2153

Mouse IL-6Rα ELISA Kit

$245.00$350.00

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity3.71 pg/mL
Array Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery89% – 112%
Average Recovery98%

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity3.71 pg/mL
Array Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery89% – 112%
Average Recovery98%
Intra Precision1.5% – 4.4%
Inter Precision1.3% - 4.2%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6Rα monoclonal antibody
Mouse IL-6Rα freeze-dried standard
IL-6Rα detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-6Rα antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6Rα present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6Rα in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL6R

IL6R Target Infomation Overview

  • Target Symbol: IL6R, interleukin 6 receptor
  • Gene Groups: CD molecules; Immunoglobulin like domain containing; Interleukin receptors
  • Alias: CD126; IL-6R; IL-1Ra; IL6RA; gp80
  • Alias Names: membrane glycoprotein 80; interleukin 6 receptor subunit alpha

IL6R, interleukin 6 receptor Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL6R Target Information

  • Target Symbol: IL6R, interleukin 6 receptor
  • Alias:
    • CD126
    • CD126 antigen
    • gp80
    • HIES5
    • IL-1Ra
    • IL-6 receptor subunit alpha
    • IL-6R
    • IL-6R 1
    • IL-6R subunit alpha
    • IL-6R-1
    • IL-6R-alpha
    • IL-6RA
    • IL6Q
    • IL6QTL
    • IL6RA
    • IL6RQ
    • interleukin 6 receptor alpha subunit
    • interleukin-6 receptor subunit alpha
    • membrane glycoprotein 80
    • MGC104991
  • NCBI_Gene: 3570
  • UniProtKB: P08887

Human IL6R Predicted Functions

Enables cytokine binding activity; enzyme binding activity; and protein homodimerization activity. Contributes to ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity; and interleukin-6 receptor binding activity. Involved in several processes, including cytokine-mediated signaling pathway; positive regulation of cell population proliferation; and positive regulation of macromolecule metabolic process. Located in apical plasma membrane and extracellular space. Part of ciliary neurotrophic factor receptor complex and interleukin-6 receptor complex. Implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Biomarker of several diseases, including Graves’ disease; obesity; proliferative diabetic retinopathy; pulmonary tuberculosis; and sleep apnea.

Mouse Il6ra Target Information

Mouse Il6ra Predicted Functions

Enables interleukin-6 binding activity and interleukin-6 receptor activity. Involved in T-helper 17 cell lineage commitment and interleukin-6-mediated signaling pathway. Located in cell surface. Part of interleukin-6 receptor complex. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system; and nervous system. Human ortholog(s) of this gene implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Orthologous to human IL6R (interleukin 6 receptor).

Rat Il6r Target Information

  • Target Symbol: Il6r, interleukin 6 receptor
  • Alias:
    • IL-6 receptor subunit alpha
    • IL-6R 1
    • IL-6R subunit alpha
    • IL-6R-alpha
    • IL-6RA
    • IL6R1
    • Il6ra
    • interleukin 6 receptor, alpha
    • interleukin-6 receptor subunit alpha
  • NCBI_Gene: 24499

Rat Il6r Predicted Functions

Predicted to enable several functions, including cytokine binding activity; cytokine receptor activity; and protein homodimerization activity. Predicted to contribute to ciliary neurotrophic factor receptor activity and interleukin-6 receptor binding activity. Involved in several processes, including response to cAMP; response to gamma radiation; and response to lipopolysaccharide. Located in extracellular space and neuronal cell body membrane. Biomarker of several diseases, including congestive heart failure; mental depression; orchitis; sciatic neuropathy; and visual epilepsy. Human ortholog(s) of this gene implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Orthologous to human IL6R (interleukin 6 receptor).

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