Mouse IL-6Rα ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL (diluent) |
| Sensitivity | 3.71 pg/mL |
| Array Range | 125 pg/mL – 8000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 89% – 112% |
| Average Recovery | 98% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL (diluent) |
| Sensitivity | 3.71 pg/mL |
| Array Range | 125 pg/mL – 8000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 89% – 112% |
| Average Recovery | 98% |
| Intra Precision | 1.5% – 4.4% |
| Inter Precision | 1.3% - 4.2% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6Rα monoclonal antibody Mouse IL-6Rα freeze-dried standard IL-6Rα detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-6Rα antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6Rα present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6Rα in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL6R
IL6R Target Infomation Overview
- Target Symbol: IL6R, interleukin 6 receptor
- Gene Groups: CD molecules; Immunoglobulin like domain containing; Interleukin receptors
- Alias: CD126; IL-6R; IL-1Ra; IL6RA; gp80
- Alias Names: membrane glycoprotein 80; interleukin 6 receptor subunit alpha
IL6R, interleukin 6 receptor Target Infomation by Species
- Human
- Mouse
- Rat
Human IL6R Target Information
- Target Symbol: IL6R, interleukin 6 receptor
- Alias:
- CD126
- CD126 antigen
- gp80
- HIES5
- IL-1Ra
- IL-6 receptor subunit alpha
- IL-6R
- IL-6R 1
- IL-6R subunit alpha
- IL-6R-1
- IL-6R-alpha
- IL-6RA
- IL6Q
- IL6QTL
- IL6RA
- IL6RQ
- interleukin 6 receptor alpha subunit
- interleukin-6 receptor subunit alpha
- membrane glycoprotein 80
- MGC104991
- NCBI_Gene: 3570
- UniProtKB: P08887
Human IL6R Predicted Functions
Enables cytokine binding activity; enzyme binding activity; and protein homodimerization activity. Contributes to ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity; and interleukin-6 receptor binding activity. Involved in several processes, including cytokine-mediated signaling pathway; positive regulation of cell population proliferation; and positive regulation of macromolecule metabolic process. Located in apical plasma membrane and extracellular space. Part of ciliary neurotrophic factor receptor complex and interleukin-6 receptor complex. Implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Biomarker of several diseases, including Graves’ disease; obesity; proliferative diabetic retinopathy; pulmonary tuberculosis; and sleep apnea.
Mouse Il6ra Target Information
- Target Symbol: Il6ra, interleukin 6 receptor, alpha
- Alias:
- CD126
- IL-6 receptor alpha chain
- IL-6R
- Il6r
- interleukin 6 receptor
- NCBI_Gene: 16194
Mouse Il6ra Predicted Functions
Enables interleukin-6 binding activity and interleukin-6 receptor activity. Involved in T-helper 17 cell lineage commitment and interleukin-6-mediated signaling pathway. Located in cell surface. Part of interleukin-6 receptor complex. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system; and nervous system. Human ortholog(s) of this gene implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Orthologous to human IL6R (interleukin 6 receptor).
Rat Il6r Target Information
- Target Symbol: Il6r, interleukin 6 receptor
- Alias:
- IL-6 receptor subunit alpha
- IL-6R 1
- IL-6R subunit alpha
- IL-6R-alpha
- IL-6RA
- IL6R1
- Il6ra
- interleukin 6 receptor, alpha
- interleukin-6 receptor subunit alpha
- NCBI_Gene: 24499
Rat Il6r Predicted Functions
Predicted to enable several functions, including cytokine binding activity; cytokine receptor activity; and protein homodimerization activity. Predicted to contribute to ciliary neurotrophic factor receptor activity and interleukin-6 receptor binding activity. Involved in several processes, including response to cAMP; response to gamma radiation; and response to lipopolysaccharide. Located in extracellular space and neuronal cell body membrane. Biomarker of several diseases, including congestive heart failure; mental depression; orchitis; sciatic neuropathy; and visual epilepsy. Human ortholog(s) of this gene implicated in Alzheimer’s disease; Huntington’s disease; hyper IgE syndrome; obesity; and stomach cancer. Orthologous to human IL6R (interleukin 6 receptor).
Products with the Same Target - IL6R
Citations for Mouse IL-6Rα ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK1316
- ELISA Kit
Human Arginase 1/ARG1 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1391
- ELISA Kit
Human ACE2 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1263
- ELISA Kit
Human 4-1BB/TNFRSF9/CD137 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK8100
- ELISA Kit
Cortisol Competitive ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1260
- ELISA Kit
Human alpha-Galactosidase A/GLA ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1275
- ELISA Kit
Human Antithrombin-III/Serpin C1 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.