Human Leptin ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	LEP
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 10 μL；cell culture supernatant: 100 μL
Sensitivity	12.9 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	87% – 112%
Average Recovery	0.98

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 10 μL；cell culture supernatant: 100 μL
Sensitivity	12.9 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	87% – 112%
Average Recovery	0.98
Intra Precision	2.7% – 4.4%
Inter-Precision	2.5% – 4.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- Leptin monoclonal antibody Human Leptin freeze-dried standard Leptin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Leptin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Leptin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Leptin in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

LEP

LEP Target Infomation Overview

Target Symbol: LEP, leptin
Gene Groups: Neuropeptides
Previous Names: OBS; OB
Alias Names: leptin (murine obesity homolog); leptin (obesity homolog, mouse)

LEP, leptin Target Infomation by Species

HumanMouseRat

Human LEP Target Information

Target Symbol: LEP, leptin
Alias:
- FLJ94114
- LEPD
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
- OB
- obese protein
- obese, mouse, homolog of
- obesity factor
- OBS
NCBI_Gene: 3952
UniProtKB: P41159

Human LEP Predicted Functions

Enables leptin receptor binding activity. Involved in several processes, including positive regulation of signal transduction; regulation of cytokine production; and regulation of lymphocyte proliferation. Predicted to be located in cytosol. Predicted to be active in extracellular space. Implicated in several diseases, including alcohol dependence; congenital leptin deficiency; liver disease (multiple); lung disease (multiple); and type 2 diabetes mellitus. Biomarker of several diseases, including alcohol dependence; cystic fibrosis; fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple).

Mouse Lep Target Information

Mouse Lep Predicted Functions

Enables DNA binding activity and growth factor activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cytokine production; and positive regulation of signal transduction. Acts upstream of or within several processes, including insulin secretion; monocarboxylic acid metabolic process; and regulation of insulin secretion. Located in cytoplasm and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; central nervous system; early conceptus; and genitourinary system. Used to study abdominal obesity-metabolic syndrome (multiple); fatty liver disease (multiple); obesity; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; liver disease (multiple); lung disease (multiple); and type 2 diabetes mellitus. Orthologous to human LEP (leptin).

Rat Lep Target Information

Target Symbol: Lep, leptin
Alias:
- OB
- obese
- obesity (murine homolog leptin)
- obesity factor
NCBI_Gene: 25608
- UniprotKB: A0A1C9CJH3
- UniprotKB: P50596

Rat Lep Predicted Functions

Enables hormone activity and peptide hormone receptor binding activity. Involved in several processes, including positive regulation of cell communication; regulation of apoptotic process; and response to vitamin. Located in cytosol and extracellular space. Used to study several diseases, including alcohol dependence; glucose metabolism disease (multiple); liver disease (multiple); neurodegenerative disease (multiple); and short bowel syndrome. Biomarker of several diseases, including Huntington’s disease; alcohol dependence; fatty liver disease (multiple); lipid metabolism disorder (multiple); and obesity. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human LEP (leptin).