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Human LIF ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.64 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 81% – 117% |
| Average Recovery | 0.97 |
| Intra Precision | 3.6%-3.8% |
| Inter-Precision | 2.0% – 4.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-LIF monoclonal antibody Human LIF freeze-dried standard LIF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human LIF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the LIF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LIF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
LIF
LIF Target Infomation Overview
- Target Symbol: LIF, LIF interleukin 6 family cytokine
- Gene Groups: Receptor ligands; Interleukin 6 type cytokine family
- Alias: CDF; DIA; HILDA
- Alias Names: differentiation inhibitory activity; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; human interleukin in DA cells; leukemia inhibitory factor; LIF, interleukin 6 family cytokine
LIF, LIF interleukin 6 family cytokine Target Infomation by Species
HumanMouseRat
Human LIF Target Information
- Target Symbol: LIF, LIF interleukin 6 family cytokine
- Alias:
- CDF
- cholinergic differentiation factor
- D factor
- DIA
- differentiation inhibitory activity
- differentiation stimulating factor
- differentiation-inducing factor
- differentiation-stimulating factor
- hepatocyte-stimulating factor III
- HILDA
- human interleukin in DA cells
- leukemia inhibitory factor
- LIF, interleukin 6 family cytokine
- melanoma-derived LPL inhibitor
- MLPLI
- NCBI_Gene: 3976
- UniProtKB: P15018
Human LIF Predicted Functions
Enables cytokine activity; growth factor activity; and leukemia inhibitory factor receptor binding activity. Involved in several processes, including leukemia inhibitory factor signaling pathway; positive regulation of protein modification process; and regulation of epithelial cell differentiation involved in kidney development. Acts upstream of or within several processes, including positive regulation of MAPK cascade; positive regulation of macrophage differentiation; and positive regulation of protein phosphorylation. Located in cytosol.
Mouse Lif Target Information
- Target Symbol: Lif, leukemia inhibitory factor
- Alias:
- 无
- NCBI_Gene: 16878
Mouse Lif Predicted Functions
Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).
Rat Lif Target Information
- Target Symbol: Lif, LIF, interleukin 6 family cytokine
- Alias:
- cholinergic neuronal differentiation factor
- leukemia inhibitory factor
- leukemia inhibitory factor (cholinergic differentiation factor)
- NCBI_Gene: 60584
Rat Lif Predicted Functions
Enables leukemia inhibitory factor receptor binding activity. Involved in several processes, including negative regulation of angiogenesis; positive regulation of corticotropin secretion; and transdifferentiation. Located in extracellular space. Biomarker of brain ischemia and congestive heart failure. Orthologous to human LIF (LIF interleukin 6 family cytokine).
