Human LIF ELISA Kit

$350.00$450.00

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TARGETSLIF
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.64 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery81% – 117%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.64 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery81% – 117%
Average Recovery0.97
Intra Precision3.6%-3.8%
Inter-Precision2.0% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-LIF monoclonal antibody
Human LIF freeze-dried standard
LIF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human LIF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the LIF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LIF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

LIF

LIF Target Infomation Overview

  • Target Symbol: LIF, LIF interleukin 6 family cytokine
  • Gene Groups: Receptor ligands; Interleukin 6 type cytokine family
  • Alias: CDF; DIA; HILDA
  • Alias Names: differentiation inhibitory activity; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; human interleukin in DA cells; leukemia inhibitory factor; LIF, interleukin 6 family cytokine

LIF, LIF interleukin 6 family cytokine Target Infomation by Species

HumanMouseRat

Human LIF Target Information

  • Target Symbol: LIF, LIF interleukin 6 family cytokine
  • Alias:
    • CDF
    • cholinergic differentiation factor
    • D factor
    • DIA
    • differentiation inhibitory activity
    • differentiation stimulating factor
    • differentiation-inducing factor
    • differentiation-stimulating factor
    • hepatocyte-stimulating factor III
    • HILDA
    • human interleukin in DA cells
    • leukemia inhibitory factor
    • LIF, interleukin 6 family cytokine
    • melanoma-derived LPL inhibitor
    • MLPLI
  • NCBI_Gene: 3976
  • UniProtKB: P15018

Human LIF Predicted Functions

Enables cytokine activity; growth factor activity; and leukemia inhibitory factor receptor binding activity. Involved in several processes, including leukemia inhibitory factor signaling pathway; positive regulation of protein modification process; and regulation of epithelial cell differentiation involved in kidney development. Acts upstream of or within several processes, including positive regulation of MAPK cascade; positive regulation of macrophage differentiation; and positive regulation of protein phosphorylation. Located in cytosol.

Mouse Lif Target Information

Mouse Lif Predicted Functions

Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).

Rat Lif Target Information

Rat Lif Predicted Functions

Enables leukemia inhibitory factor receptor binding activity. Involved in several processes, including negative regulation of angiogenesis; positive regulation of corticotropin secretion; and transdifferentiation. Located in extracellular space. Biomarker of brain ischemia and congestive heart failure. Orthologous to human LIF (LIF interleukin 6 family cytokine).

LIF Target News

    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis

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