EK1358

Human LYVE-1 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity2.01 pg/mL
Array Range31.22 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery86% – 105%
Average Recovery95%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity2.01 pg/mL
Array Range31.22 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery86% – 105%
Average Recovery95%
Intra Precision2.9% – 3.8%
Inter Precision3.8% - 4.9%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- LYVE-1 monoclonal antibody
Human LYVE-1 freeze-dried standard
LYVE-1 detect Antibody
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human LYVE-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, LYVE-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LYVE-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

LYVE1

LYVE1 Target Infomation Overview

  • Target Symbol: LYVE1, lymphatic vessel endothelial hyaluronan receptor 1
  • Alias: LYVE-1
  • Previous Names: XLKD1
  • Alias Names: extracellular link domain containing 1

LYVE1, lymphatic vessel endothelial hyaluronan receptor 1 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human LYVE1 Target Information

  • Target Symbol: LYVE1, lymphatic vessel endothelial hyaluronan receptor 1
  • Alias:
    • cell surface retention sequence binding protein-1
    • cell surface retention sequence-binding protein 1
    • CRSBP-1
    • extracellular link domain containing 1
    • extracellular link domain-containing 1
    • extracellular link domain-containing protein 1
    • HAR
    • hyaluronic acid receptor
    • lymphatic vessel endothelial hyaluronic acid receptor 1
    • LYVE-1
    • XLKD1
  • NCBI_Gene: 10894
  • UniProtKB: Q9Y5Y7

Human LYVE1 Predicted Functions

Predicted to enable hyaluronic acid binding activity and transmembrane signaling receptor activity. Predicted to be involved in anatomical structure morphogenesis; cell-matrix adhesion; and response to wounding. Predicted to act upstream of or within glycosaminoglycan catabolic process. Located in extracellular exosome.

Mouse Lyve1 Target Information

  • Target Symbol: Lyve1, lymphatic vessel endothelial hyaluronan receptor 1
  • Alias:
    • 1200012G08Rik
    • extra cellular link domain-containing 1
    • lymphatic vessel endothelial HA receptor-1
    • Lyve-1
    • RIKEN cDNA 1200012G08 gene
    • Xlkd1
  • NCBI_Gene: 114332

Mouse Lyve1 Predicted Functions

Enables hyaluronic acid binding activity and transmembrane signaling receptor activity. Acts upstream of or within glycosaminoglycan catabolic process. Located in plasma membrane. Is expressed in several structures, including cardiovascular system; gut; hemolymphoid system; meninges; and metanephros. Orthologous to human LYVE1 (lymphatic vessel endothelial hyaluronan receptor 1).

Rat Lyve1 Target Information

  • Target Symbol: Lyve1, lymphatic vessel endothelial hyaluronan receptor 1
  • Alias:
    • extra cellular link domain-containing 1
    • extracellular link domain-containing 1
    • LOC293186
    • lymphatic vessel endothelial hyaluronic acid receptor 1
    • Xlkd1
  • NCBI_Gene: 293186
  • UniProtKB: D3ZD19

Rat Lyve1 Predicted Functions

Predicted to enable hyaluronic acid binding activity and transmembrane signaling receptor activity. Predicted to be involved in cell adhesion. Predicted to act upstream of or within glycosaminoglycan catabolic process. Predicted to be located in cell periphery. Predicted to be active in plasma membrane. Orthologous to human LYVE1 (lymphatic vessel endothelial hyaluronan receptor 1).

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