Human M-CSF ELISA?Kit
$245.00 – $350.00
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 20 μL |
Sensitivity | 0.19 pg/mL |
Array Range | 31.25 pg/mL – 2000 pg/mL |
Assay Time | 3.5 h |
Recovery | 81% – 116% |
Average Recovery | 102% |
ELISA Kit Detail Information
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 20 μL |
Sensitivity | 0.19 pg/mL |
Array Range | 31.25 pg/mL – 2000 pg/mL |
Assay Time | 3.5 h |
Recovery | 81% – 116% |
Average Recovery | 102% |
Intra Precision | 2.1% – 4.1% |
Inter Precision | 3.1% – 4.0% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-M-CSF monoclonal antibody Human M-CSF freeze-dried standard M-CSF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human M-CSF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the M-CSF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of M-CSF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
CSF1
CSF1 Target Infomation Overview
- Target Symbol: CSF1, colony stimulating factor 1
- Alias: M-CSF; MCSF; MGC31930
- Alias Names: macrophage colony stimulating factor 1; colony stimulating factor 1 (macrophage)
CSF1, colony stimulating factor 1 Target Infomation by Species
- Human
- Mouse
- Rat
Human CSF1 Target Information
- Target Symbol: CSF1, colony stimulating factor 1
- Alias:
- colony stimulating factor 1 (macrophage)
- CSF-1
- lanimostim
- macrophage colony stimulating factor
- macrophage colony stimulating factor 1
- macrophage colony-stimulating factor 1
- MCSF
- MGC31930
- NCBI_Gene: 1435
- UniProtKB: P09603
Human CSF1 Predicted Functions
Enables cytokine activity; growth factor activity; and protein homodimerization activity. Involved in several processes, including positive regulation of cell differentiation; positive regulation of macromolecule metabolic process; and positive regulation of macrophage migration. Acts upstream of or within positive regulation of cell population proliferation. Located in several cellular components, including extracellular space; nuclear body; and perinuclear region of cytoplasm. Implicated in interstitial cystitis. Biomarker of several diseases, including COVID-19; atherosclerosis; end stage renal disease; glomerulonephritis (multiple); and vesicoureteral reflux.
Mouse Csf1 Target Information
- Target Symbol: Csf1, colony stimulating factor 1 (macrophage)
- Alias:
- C87615
- colony stimulating factor, macrophage
- colony-stimulating factor-1
- CSF-1
- Csfm
- expressed sequence C87615
- M-CSF
- op
- osteopetrosis
- NCBI_Gene: 12977
Mouse Csf1 Predicted Functions
Enables macrophage colony-stimulating factor receptor binding activity and protein homodimerization activity. Involved in several processes, including macrophage colony-stimulating factor signaling pathway; microglial cell proliferation; and positive regulation of gene expression. Acts upstream of or within several processes, including mammary gland development; osteoclast proliferation; and positive regulation of myeloid leukocyte differentiation. Located in extracellular space. Part of CSF1-CSF1R complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and hindlimb long bone. Used to study osteopetrosis. Human ortholog(s) of this gene implicated in interstitial cystitis. Orthologous to human CSF1 (colony stimulating factor 1).
Rat Csf1 Target Information
- Target Symbol: Csf1, colony stimulating factor 1
- Alias:
- colony stimulating factor 1 (macrophage)
- colony stimulating factor 1(macrophage)
- CSF-1
- macrophage colony-stimulating factor 1
- MCSF
- NCBI_Gene: 78965
Rat Csf1 Predicted Functions
Enables identical protein binding activity. Involved in several processes, including myeloid leukocyte differentiation; odontogenesis; and ossification. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; high grade glioma; and osteopetrosis. Biomarker of systemic scleroderma. Human ortholog(s) of this gene implicated in interstitial cystitis. Orthologous to human CSF1 (colony stimulating factor 1).
Citations for Human M-CSF ELISA?Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Reviews
There are no reviews yet.