Human MMP-1 ELISA Kit

$350.00$450.00

Download:
TARGETSMMP1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity11.19 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery83% – 115%
Average Recovery1.07
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity11.19 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery83% – 115%
Average Recovery1.07
Intra Precision2.9% – 4.9%
Inter-Precision3.9% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-1 monoclonal antibody
Human MMP-1 freeze-dried standard
MMP-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-1 in the samples. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MMP1

MMP1 Target Infomation Overview

  • Target Symbol: MMP1, matrix metallopeptidase 1
  • Gene Groups: Receptor ligands; M10 matrix metallopeptidases
  • Previous Names: CLG
  • Alias Names: interstitial collagenase; matrix metalloproteinase 1 (interstitial collagenase)

MMP1, matrix metallopeptidase 1 Target Infomation by Species

HumanMouseRat

Human MMP1 Target Information

  • Target Symbol: MMP1, matrix metallopeptidase 1
  • Alias:
    • CLG
    • CLGN
    • fibroblast collagenase
    • interstitial collagenase
    • matrix metallopeptidase 1 (interstitial collagenase)
    • matrix metalloprotease 1
    • matrix metalloproteinase 1
    • matrix metalloproteinase 1 (interstitial collagenase)
  • NCBI_Gene: 4312
  • UniProtKB: P03956

Human MMP1 Predicted Functions

Enables metalloendopeptidase activity. Involved in cellular response to UV-A; positive regulation of protein-containing complex assembly; and proteolysis. Predicted to be located in extracellular region. Implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Biomarker of several diseases, including artery disease (multiple); arthritis (multiple); idiopathic pulmonary fibrosis; immunoglobulin light chain amyloidosis; and systemic lupus erythematosus.

Mouse Mmp1a Target Information

Mouse Mmp1a Predicted Functions

Predicted to enable metalloendopeptidase activity. Predicted to be involved in collagen catabolic process and extracellular matrix organization. Is expressed in several structures, including extraembryonic component and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Orthologous to human MMP1 (matrix metallopeptidase 1).

Rat Mmp1 Target Information

  • Target Symbol: Mmp1, matrix metallopeptidase 1
  • Alias:
    • Clgn
    • fibroblast collagenase
    • interstitial collagenase
    • LOC300339
    • matrix metallopeptidase 1 (interstitial collagenase)
    • matrix metallopeptidase 1a
    • matrix metallopeptidase 1a (interstitial collagenase)
    • matrix metalloproteinase 1
    • matrix metalloproteinase 1a (interstitial collagenase)
    • matrix metalloproteinase-1
    • MMP-1
    • Mmp1a
    • myocardial collagenase
  • NCBI_Gene: 300339

Rat Mmp1 Predicted Functions

Enables peptidase activity. Involved in several processes, including cellular response to cytokine stimulus; female gonad development; and positive regulation of cell migration. Located in cytoplasm and extracellular space. Used to study several diseases, including artery disease (multiple); degenerative disc disease; liver cirrhosis; pulmonary fibrosis; and ulcerative colitis. Biomarker of several diseases, including chronic obstructive pulmonary disease; liver cirrhosis; scoliosis; tendinitis; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Orthologous to human MMP1 (matrix metallopeptidase 1).

MMP1 Target News

    5.8
    Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes
    BIOCHEMICAL PHARMACOLOGY
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Osteoarthrosis & Rheumatoid Arthritis
    Tumor Marker

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    Same Target