Human MMP-1 ELISA Kit

$245.00 – $350.00

Related Target	MMP1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	11.19 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	83% – 115%
Average Recovery	107%

ELISA Kit Detail Information

Related Target	MMP1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	11.19 pg/mL
Array Range	0.16 ng/mL – 10 ng/mL
Assay Time	3.5 h
Recovery	83% – 115%
Average Recovery	107%
Intra Precision	2.9% – 4.9%
Inter Precision	3.9% – 4.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-1 monoclonal antibody Human MMP-1 freeze-dried standard MMP-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-1 in the samples. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

MMP1

MMP1 Target Infomation Overview

Target Symbol: MMP1, matrix metallopeptidase 1
Gene Groups: Receptor ligands; M10 matrix metallopeptidases
Previous Names: CLG
Alias Names: interstitial collagenase; matrix metalloproteinase 1 (interstitial collagenase)

MMP1, matrix metallopeptidase 1 Target Infomation by Species

Human
Mouse
Rat

Human MMP1 Target Information

Target Symbol: MMP1, matrix metallopeptidase 1
Alias:
- CLG
- CLGN
- fibroblast collagenase
- interstitial collagenase
- matrix metallopeptidase 1 (interstitial collagenase)
- matrix metalloprotease 1
- matrix metalloproteinase 1
- matrix metalloproteinase 1 (interstitial collagenase)
NCBI_Gene: 4312
UniProtKB: P03956

Human MMP1 Predicted Functions

Enables metalloendopeptidase activity. Involved in cellular response to UV-A; positive regulation of protein-containing complex assembly; and proteolysis. Predicted to be located in extracellular region. Implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Biomarker of several diseases, including artery disease (multiple); arthritis (multiple); idiopathic pulmonary fibrosis; immunoglobulin light chain amyloidosis; and systemic lupus erythematosus.

Mouse Mmp1a Target Information

Target Symbol: Mmp1a, matrix metallopeptidase 1a (interstitial collagenase)
Alias:
- Mcol-A
NCBI_Gene: 83995
- UniprotKB: Q9EPL5

Mouse Mmp1a Predicted Functions

Predicted to enable metalloendopeptidase activity. Predicted to be involved in collagen catabolic process and extracellular matrix organization. Is expressed in several structures, including extraembryonic component and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Orthologous to human MMP1 (matrix metallopeptidase 1).

Rat Mmp1 Target Information

Target Symbol: Mmp1, matrix metallopeptidase 1
Alias:
- Clgn
- fibroblast collagenase
- interstitial collagenase
- LOC300339
- matrix metallopeptidase 1 (interstitial collagenase)
- matrix metallopeptidase 1a
- matrix metallopeptidase 1a (interstitial collagenase)
- matrix metalloproteinase 1
- matrix metalloproteinase 1a (interstitial collagenase)
- matrix metalloproteinase-1
- MMP-1
- Mmp1a
- myocardial collagenase
NCBI_Gene: 300339
- UniprotKB: B5DFD5
- UniprotKB: P81563

Rat Mmp1 Predicted Functions

Enables peptidase activity. Involved in several processes, including cellular response to cytokine stimulus; female gonad development; and positive regulation of cell migration. Located in cytoplasm and extracellular space. Used to study several diseases, including artery disease (multiple); degenerative disc disease; liver cirrhosis; pulmonary fibrosis; and ulcerative colitis. Biomarker of several diseases, including chronic obstructive pulmonary disease; liver cirrhosis; scoliosis; tendinitis; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Orthologous to human MMP1 (matrix metallopeptidase 1).

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