Human MMP-2 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	MMP2
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	69.14 pg/mL
Range	2 ng/mL – 128 ng/mL
Assay Time	3.5 h
Recovery	86% – 118%
Average Recovery	1.08

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	69.14 pg/mL
Range	2 ng/mL – 128 ng/mL
Assay Time	3.5 h
Recovery	86% – 118%
Average Recovery	1.08
Intra Precision	2.1% – 7.8%
Inter-Precision	2.4% – 3.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-2 monoclonal antibody Human MMP-2 freeze-dried standard MMP-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-2 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

MMP2

MMP2 Target Infomation Overview

Target Symbol: MMP2, matrix metallopeptidase 2
Gene Groups: M10 matrix metallopeptidases
Alias: TBE-1
Previous Names: CLG4; CLG4A
Alias Names: matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

MMP2, matrix metallopeptidase 2 Target Infomation by Species

HumanMouseRat

Human MMP2 Target Information

Target Symbol: MMP2, matrix metallopeptidase 2
Alias:
- 72 kDa gelatinase
- 72 kDa type IV collagenase
- CLG4
- CLG4A
- collagenase type IV-A
- gelatinase A
- matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
- matrix metalloproteinase 2 (gelatinase a, 72kda gelatinase, 72kda type iv collagenase)
- matrix metalloproteinase-2
- matrix metalloproteinase-II
- MMP-2
- MMP-II
- MONA
- neutrophil gelatinase
- TBE-1
NCBI_Gene: 4313
UniProtKB: P08253

Human MMP2 Predicted Functions

Enables metallopeptidase activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; endodermal cell differentiation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Biomarker of several diseases, including artery disease (multiple); carcinoma (multiple); cardiomyopathy (multiple); cholesteatoma (multiple); and neurodegenerative disease (multiple).

Mouse Mmp2 Target Information

Target Symbol: Mmp2, matrix metallopeptidase 2
Alias:
- 72kDa gelatinase
- 72kDa type IV collagenase
- Clg4a
- collagenase IVA, basement membrane, 72 kDa
- GelA
- gelatinase A
- MMP-2
NCBI_Gene: 17390

Mouse Mmp2 Predicted Functions

Enables metalloendopeptidase activity. Involved in response to amyloid-beta. Acts upstream of or within several processes, including blood vessel maturation; bone trabecula formation; and collagen catabolic process. Located in several cellular components, including extracellular space; mitochondrion; and sarcomere. Is expressed in several structures, including embryo mesenchyme; genitourinary system; heart; jaw; and musculoskeletal system. Human ortholog(s) of this gene implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Orthologous to human MMP2 (matrix metallopeptidase 2).

Rat Mmp2 Target Information

Target Symbol: Mmp2, matrix metallopeptidase 2
Alias:
- 72 kDa gelatinase
- 72 KDa type IV collagenase
- gelatinase A
- matrix metalloproteinase 2
- matrix metalloproteinase 2 (72 KDa type IV collagenase)
- matrix metalloproteinase-2
- MMP-2
NCBI_Gene: 81686
- UniprotKB: E9PSM5
- UniprotKB: P33436

Rat Mmp2 Predicted Functions

Enables fibronectin binding activity and peptidase activity. Involved in several processes, including cellular response to estradiol stimulus; circulatory system development; and reproductive structure development. Located in cytoplasm and extracellular space. Used to study artery disease (multiple); conjunctivitis; and transient cerebral ischemia. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); bile duct disease (multiple); brain ischemia (multiple); and liver cirrhosis (multiple). Human ortholog(s) of this gene implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Orthologous to human MMP2 (matrix metallopeptidase 2).