Human MMP-2 ELISA Kit

$350.00$450.00

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TARGETSMMP2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity69.14 pg/mL
Range2 ng/mL – 128 ng/mL
Assay Time3.5 h
Recovery86% – 118%
Average Recovery1.08
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity69.14 pg/mL
Range2 ng/mL – 128 ng/mL
Assay Time3.5 h
Recovery86% – 118%
Average Recovery1.08
Intra Precision2.1% – 7.8%
Inter-Precision2.4% – 3.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-2 monoclonal antibody
Human MMP-2 freeze-dried standard
MMP-2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-2 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MMP2

MMP2 Target Infomation Overview

  • Target Symbol: MMP2, matrix metallopeptidase 2
  • Gene Groups: M10 matrix metallopeptidases
  • Alias: TBE-1
  • Previous Names: CLG4; CLG4A
  • Alias Names: matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

MMP2, matrix metallopeptidase 2 Target Infomation by Species

HumanMouseRat

Human MMP2 Target Information

  • Target Symbol: MMP2, matrix metallopeptidase 2
  • Alias:
    • 72 kDa gelatinase
    • 72 kDa type IV collagenase
    • CLG4
    • CLG4A
    • collagenase type IV-A
    • gelatinase A
    • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
    • matrix metalloproteinase 2 (gelatinase a, 72kda gelatinase, 72kda type iv collagenase)
    • matrix metalloproteinase-2
    • matrix metalloproteinase-II
    • MMP-2
    • MMP-II
    • MONA
    • neutrophil gelatinase
    • TBE-1
  • NCBI_Gene: 4313
  • UniProtKB: P08253

Human MMP2 Predicted Functions

Enables metallopeptidase activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; endodermal cell differentiation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Biomarker of several diseases, including artery disease (multiple); carcinoma (multiple); cardiomyopathy (multiple); cholesteatoma (multiple); and neurodegenerative disease (multiple).

Mouse Mmp2 Target Information

Mouse Mmp2 Predicted Functions

Enables metalloendopeptidase activity. Involved in response to amyloid-beta. Acts upstream of or within several processes, including blood vessel maturation; bone trabecula formation; and collagen catabolic process. Located in several cellular components, including extracellular space; mitochondrion; and sarcomere. Is expressed in several structures, including embryo mesenchyme; genitourinary system; heart; jaw; and musculoskeletal system. Human ortholog(s) of this gene implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Orthologous to human MMP2 (matrix metallopeptidase 2).

Rat Mmp2 Target Information

  • Target Symbol: Mmp2, matrix metallopeptidase 2
  • Alias:
    • 72 kDa gelatinase
    • 72 KDa type IV collagenase
    • gelatinase A
    • matrix metalloproteinase 2
    • matrix metalloproteinase 2 (72 KDa type IV collagenase)
    • matrix metalloproteinase-2
    • MMP-2
  • NCBI_Gene: 81686

Rat Mmp2 Predicted Functions

Enables fibronectin binding activity and peptidase activity. Involved in several processes, including cellular response to estradiol stimulus; circulatory system development; and reproductive structure development. Located in cytoplasm and extracellular space. Used to study artery disease (multiple); conjunctivitis; and transient cerebral ischemia. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); bile duct disease (multiple); brain ischemia (multiple); and liver cirrhosis (multiple). Human ortholog(s) of this gene implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Orthologous to human MMP2 (matrix metallopeptidase 2).

MMP2 Target News

    2.3
    Inhibition of LAMP3 mediates the protective effect of vitamin D against hypoxia/reoxygenation in trophoblast cells
    BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
    5
    Oncogenic Role of the NFATC2/NEDD4/FBP1 Axis in Cholangiocarcinoma
    LABORATORY INVESTIGATION
    5.8
    Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes
    BIOCHEMICAL PHARMACOLOGY
    6
    Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis
    AMERICAN JOURNAL OF PATHOLOGY
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Tumor Marker

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